Wolfram L, Eitinger T, Friedrich B
Institut für Pflanzenphysiologie und Mikrobiologie, Freien Universität Berlin, Germany.
FEBS Lett. 1991 May 20;283(1):109-12. doi: 10.1016/0014-5793(91)80565-k.
The high-affinity nickel transporter of Alcaligenes eutrophus H16 is encoded by gene hoxN, which maps within the hydrogenase gene cluster of megaplasmid pHG1. A tripartite gene fusion was constructed, consisting of (i) the Escherichia coli lacZ gene for beta-galactosidase, (ii) a segment encoding an endoproteolytically cleavable peptide, and (iii) the A. eutrophus gene hoxN. An E. coli strain harboring this construct (plasmid pCH307) efficiently produced the corresponding triprotein upon induction. A broad-host-range derivative of pCH307 was shown to complement an A. eutrophus HoxN- mutant.
