Department of Biological Engineering, Inha University, Incheon, Korea.
J Microbiol Biotechnol. 2010 Mar;20(3):480-4.
AfsR2 is a global regulatory protein which stimulates antibiotic biosynthesis in both Streptomyces lividans and S. coelicolor. Previously, various afsR2-dependent genes including a putative abaA-like regulatory gene, SCO4677, were identified through comparative DNA microarray analysis. To further identify the putative SCO4677-dependent proteins, the comparative proteomics-driven approach was applied to the SCO4677-overexpressing strains of S. lividans and S. coelicolor along with the wild-type strains. The 2D gel-electrophoresis gave approximately 277 protein spots for S. lividans and 207 protein spots for S. coelicolor, showing different protein expression patterns between the SCO4677-overexpressing strains and the wild-type strains. Further MALDI-TOF analysis revealed that only 18 proteins exhibited similar expression patterns in both S. lividans and S. coelicolor, suggesting that the SCO4677 could encode an abaA-like regulator which controls a few cross-species proteins as well as many species-specific proteins in Streptomyces species.
AfsR2 是一种全局调控蛋白，可刺激链霉菌属中的链霉菌属和变铅青链霉菌的抗生素生物合成。先前，通过比较 DNA 微阵列分析鉴定了各种依赖 afsR2 的基因，包括一个假定的 abaA 样调控基因 SCO4677。为了进一步鉴定假定的 SCO4677 依赖性蛋白，应用比较蛋白质组学驱动的方法对链霉菌属和变铅青链霉菌属的 SCO4677 过表达菌株以及野生型菌株进行了研究。2D 凝胶电泳为链霉菌属提供了大约 277 个蛋白斑点，为变铅青链霉菌属提供了 207 个蛋白斑点，显示了 SCO4677 过表达菌株与野生型菌株之间不同的蛋白表达模式。进一步的 MALDI-TOF 分析表明，只有 18 种蛋白在链霉菌属和变铅青链霉菌属中表现出相似的表达模式，这表明 SCO4677 可以编码一种 abaA 样调控因子，该因子控制一些种间蛋白以及链霉菌属中的许多种特异性蛋白。
