Department of Human and General Physiology, Alma Mater Studiorum-University of Bologna, Bologna, Italy.
J Sleep Res. 2010 Sep;19(3):394-9. doi: 10.1111/j.1365-2869.2009.00810.x. Epub 2010 Mar 31.
In different species, rapid eye movement sleep (REMS) is characterized by a thermoregulatory impairment. It has been postulated that this impairment depends on a general insufficiency in the hypothalamic integration of autonomic function. This study aims to test this hypothesis by assessing the hypothalamic regulation of body fluid osmolality during the different wake-sleep states in the rat. Arginine-vasopressin (AVP) plasma levels were determined following intracerebroventricular (ICV) infusions of artificial cerebrospinal fluid (aCSF), either isotonic or made hypertonic by the addition of NaCl at three different concentrations (125, 250 and 500 mM). Animals were implanted with a cannula within a lateral cerebral ventricle for ICV infusions and with electrodes for the recording of the electroencephalogram. ICV infusions were made in different animals during Wake, REMS or non-REM sleep (NREMS). The results show that ICV infusion of hypertonic aCSF during REMS induced an increase in AVP plasma levels that was not different from that observed during either Wake or NREMS. These results suggest that the thermoregulatory impairment that characterizes REMS does not depend on a general impairment in the hypothalamic control of body homeostasis.
在不同物种中，快速眼动睡眠（REMS）的特征是体温调节受损。据推测，这种损伤取决于下丘脑对自主功能整合的普遍不足。本研究旨在通过评估大鼠在不同清醒-睡眠状态下体液渗透压的下丘脑调节来检验这一假设。通过向侧脑室内（ICV）输注人工脑脊液（aCSF），确定精氨酸加压素（AVP）的血浆水平，通过向 aCSF 中添加三种不同浓度（125、250 和 500mM）的 NaCl 使其分别达到等渗或高渗。动物在接受 ICV 输注和记录脑电图的电极植入后，进行了侧脑室内的套管植入。在 Wake、REMS 或非快速眼动睡眠（NREMS）期间，在不同动物中进行了 ICV 输注。结果表明，在 REMS 期间，ICV 输注高渗 aCSF 会引起 AVP 血浆水平升高，这与在 Wake 或 NREMS 期间观察到的水平没有差异。这些结果表明，快速眼动睡眠特征的体温调节受损并不依赖于下丘脑对身体内稳态的控制的普遍受损。
