Laboratory of Water Science & Technology, School of Life Science & Biotechnology, Shanghai Jiaotong University, Shanghai 200240, China.
J Water Health. 2010 Sep;8(3):593-600. doi: 10.2166/wh.2010.048. Epub 2010 Mar 9.
Community diversity and abundance of biofilms from a full-scale drinking water distribution system in Shanghai were characterized by denaturing gradient gel electrophoresis (DGGE) analysis of 16S rRNA sequences and heterotrophic plate count (HPC), respectively. Bacteria affiliated to the Beta- and Gamma-Proteobacteria were dominating in both in-situ and HPC-culturable bacterial communities. Other bacteria present included members of Alphaproteobacteria, Bacteroides, Actinobacteria, Nitrospirae and Firmicutes. Acidovorax, Ralstonia and Acinetobacter were common species in biofilms. Klebsiella pneumoniae and Enterobacter sp. were detected in the local distribution system. Dissolved organic carbon (DOC), residual disinfectant and temperature were the most important factors influencing both bacterial abundance and composition. HPC for biofilm sample was not correlated with its community diversity.
采用变性梯度凝胶电泳（DGGE）分析 16S rRNA 序列和异养平板计数（HPC）的方法，分别对上海一个全规模饮用水分配系统中的群落多样性和生物膜丰度进行了研究。原位和可培养的细菌群落中，β-和γ-变形菌门的细菌占主导地位。其他存在的细菌包括α-变形菌门、拟杆菌门、放线菌门、硝化螺旋菌门和厚壁菌门的成员。食酸菌属、罗尔斯通氏菌属和不动杆菌属是生物膜中的常见物种。在当地的分配系统中检测到肺炎克雷伯菌和肠杆菌属。溶解有机碳（DOC）、残留消毒剂和温度是影响细菌丰度和组成的最重要因素。生物膜样本的 HPC 与群落多样性没有相关性。
