Artemin可提高螺旋神经节神经元在体内和体外的存活率。
Artemin improves survival of spiral ganglion neurons in vivo and in vitro.
作者信息
Warnecke Athanasia, Scheper Verena, Buhr Ines, Wenzel Gentiana I, Wissel Kirsten, Paasche Gerrit, Berkingali Nurdanat, Jørgensen Jesper Roland, Lenarz Thomas, Stöver Timo
机构信息
Department of Otolaryngology, Head and Neck Surgery, Hannover Medical School, Hannover, Germany.
出版信息
Neuroreport. 2010 May 12;21(7):517-21. doi: 10.1097/WNR.0b013e328339045b.
Artemin and its receptors are upregulated in the auditory nerve of deafened rats as a possible intrinsic protective mechanism against ototoxicity-related apoptosis. Consequently, we examined the effect of artemin on spiral ganglion neurons in vitro and in vivo. Spiral ganglion neurons were isolated from neonatal rats and cultured in serum-free medium supplemented with artemin and/or brain-derived neurotrophic factor (BDNF). In vitro, the survival rate of spiral ganglion neurons cultivated with artemin or BDNF was significantly improved compared with negative controls. In addition, artemin was delivered to the inner ear of deafened guinea pigs for 28 days. In-vivo artemin was as effective as BDNF in spiral ganglion neuron protection. Therefore, artemin promotes the survival of spiral ganglion neurons in vitro and in vivo.
在耳聋大鼠的听神经中，Artemin及其受体上调，这可能是一种针对耳毒性相关凋亡的内在保护机制。因此，我们在体外和体内研究了Artemin对螺旋神经节神经元的作用。从新生大鼠分离出螺旋神经节神经元，并在补充了Artemin和/或脑源性神经营养因子（BDNF）的无血清培养基中培养。在体外，与阴性对照相比，用Artemin或BDNF培养的螺旋神经节神经元的存活率显著提高。此外，将Artemin注入耳聋豚鼠内耳28天。在体内，Artemin在保护螺旋神经节神经元方面与BDNF一样有效。因此，Artemin在体外和体内均能促进螺旋神经节神经元的存活。
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