Suzuki T, Watanabe S
Tama Branch Laboratory, Tokyo Metropolitan Research Laboratory of Public Health, Japan.
J Chromatogr. 1991 Mar 22;541(1-2):359-64. doi: 10.1016/s0021-9673(01)96007-8.
A high-performance liquid chromatographic (HPLC) method for phenoxy acid herbicides using precolumn derivatization with 9-anthryldiazomethane (ADAM) is presented. The phenoxy acid herbicides investigated were (2,4-dichlorophenoxy)acetic acid, (4-chloro-2-methylphenoxy)acetic acid, 2-(4-chloro-2-methylphenoxy)propionic acid and (4-chloro-2-methylphenoxy)butyric acid. These herbicides reacted with ADAM under mild conditions and were converted into the corresponding fluorescent derivatives. The ADAM derivatives were separated by reversed-phase HPLC and determined using a fluorescence detector. The detection limits were about 500 pg per injection. For the application of ADAM to the determination of these herbicides in ground waters, the recoveries were more than 93% and the average relative standard deviation was 6.0% at 0.5 microgram/l. The procedure is useful as a screening method for phenoxy acid herbicides in ground water samples.
本文介绍了一种采用9-蒽重氮甲烷（ADAM）柱前衍生化的高效液相色谱（HPLC）法测定苯氧羧酸类除草剂。所研究的苯氧羧酸类除草剂包括（2,4-二氯苯氧基）乙酸、（4-氯-2-甲基苯氧基）乙酸、2-（4-氯-2-甲基苯氧基）丙酸和（4-氯-2-甲基苯氧基）丁酸。这些除草剂在温和条件下与ADAM反应，转化为相应的荧光衍生物。ADAM衍生物通过反相HPLC分离，并用荧光检测器测定。检测限约为每次进样500 pg。对于将ADAM应用于测定地下水中的这些除草剂，在0.5微克/升时回收率超过93%，平均相对标准偏差为6.0%。该方法可作为地下水样品中苯氧羧酸类除草剂的筛选方法。
