Key Laboratory of Swine Genetics and Breeding, Ministry of Agriculture, College of Animal Science and Veterinary Medicine, Huazhong Agricultural University, Wuhan, 430070, People's Republic of China.
Mol Biol Rep. 2011 Feb;38(2):827-32. doi: 10.1007/s11033-010-0172-5. Epub 2010 Apr 8.
In this study, the expression profiling of three troponin I isoforms (TNNI1, TNNI2 and TNNI3) was investigated in two pig breeds differing in muscularity (Yorkshire and Meishan) at six stages (fetal 60 days and postnatal 3, 35, 60, 120, and 180 days) and three types of muscles (longissimus dorsi muscle, LD; semitendinosus, ST; cardiac muscle, CM) using relative real-time quantitative PCR. Significant differences of troponin I expression in three muscles were found between Yorkshire and Meishan breeds at some stages. The expression peak of TNNI1 and TNNI2 in LD and ST was at postnatal 35 or 60 days in Yorkshire and at postnatal 120 or 180 days in Meishan pigs, while it occurred in CM at postnatal 3 days in two pig breeds. The relative expression values of TNNI1 and TNNI2 were significantly higher in LD than ST at most of stages after birth. The expression ratio of TNNI2 versus TNNI1 favoured TNNI2 expression in ST and LD, but on the contrary in CM. The expression peak of TNNI3 occurred at postnatal 60 and 120 days in Yorkshire and Meishan pigs, respectively. TNNI1 and TNNI3 were co-expressed in CM during the fetal and earlier stages after birth.
本研究采用相对实时定量 PCR 技术，研究了在肌肉发达程度不同的两个猪品种（约克夏和梅山）的六个阶段（胎儿 60 天和出生后 3、35、60、120 和 180 天）和三种肌肉（背最长肌、半腱肌、心肌）中三种肌钙蛋白 I 同工型（TNNI1、TNNI2 和 TNNI3）的表达谱。在某些阶段，约克夏和梅山品种之间三种肌肉的肌钙蛋白 I 表达存在显著差异。TNNI1 和 TNNI2 在 LD 和 ST 的表达高峰在约克夏出生后 35 或 60 天，在梅山猪出生后 120 或 180 天，而在两种猪中均在 CM 出生后 3 天。TNNI1 和 TNNI2 的相对表达值在出生后大多数阶段均显著高于 LD 中的 ST。TNNI2 与 TNNI1 的表达比值有利于 ST 和 LD 中的 TNNI2 表达，但在 CM 中则相反。TNNI3 的表达高峰分别出现在约克夏和梅山猪出生后 60 和 120 天。TNNI1 和 TNNI3 在胎儿和出生后早期阶段在 CM 中共同表达。
