[锌指核酸酶及其应用]
[Zinc finger nucleases and their application].
作者信息
Deng Shan-shan, Wang Ying-zhi, Ma Duan
机构信息
Key Laboratory of Molecular Medicine, Ministry of Education, Shanghai Medical College, Fudan University, Shanghai, P.R. China.
出版信息
Zhonghua Yi Xue Yi Chuan Xue Za Zhi. 2010 Apr;27(2):162-5. doi: 10.3760/cma.j.issn.1003-9406.2010.02.010.
Zinc finger nuclease (ZFN), which is a chimeric fusion structure between a Cys2-His2 zinc-finger protein (ZFP) and the cleavage domain of Fok I endonuclease, can be used to introduce targeted double-stranded breaks (DSBs). ZFN-mediated cleavage leads to mutations when double-stranded breaks are repaired by homologous recombination (HR) or nonhomologous end joining (NHEJ). In recent years, ZFNs are widely used in the fields of genetic research. In this review, the methodology and technical advantages of ZFNs were briefly discussed.
锌指核酸酶（ZFN）是一种在Cys2-His2锌指蛋白（ZFP）与Fok I核酸内切酶的切割结构域之间的嵌合融合结构，可用于引入靶向双链断裂（DSB）。当双链断裂通过同源重组（HR）或非同源末端连接（NHEJ）修复时，ZFN介导的切割会导致突变。近年来，ZFNs在基因研究领域得到广泛应用。在本综述中，简要讨论了ZFNs的方法和技术优势。
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