Department of Veterinary Physiology and Pharmacology, Ahmadu Bello University, Zaria, Nigeria.
Hum Exp Toxicol. 2011 Jan;30(1):19-24. doi: 10.1177/0960327110368415. Epub 2010 Apr 8.
Chronic exposure to chlorpyrifos (CPF) has been shown to cause increased lipoperoxidative changes in the erythrocyte membranes. The relationship between chronic CPF-induced lipoperoxidative changes and erythrocyte fragility has not been elucidated. The aim of the present study is to evaluate the role of lipoperoxidation on CPF-induced erythrocyte fragility and the ameliorative effect of vitamin C. Twenty animals divided at random into four groups of five animals each served as subject for this study. Rats in group I served as the control group and were given only soya oil at a dose of 2 mL/kg body weight (b.w.). Rats in group II were dosed with vitamin C (100 mg/kg b.w.) and then supplemented with soya oil (2 mL/kg b.w.), while those in group III were administered with CPF only at a dose of 10.6 mg/kg b.w. (~one-eighth of the previously determined median lethal dose [LD(50)]). Rats in group IV were pretreated with 100 mg/kg b.w. of vitamin C, and then dosed with CPF at a dose of 10.6 mg/kg b.w., 30 min later. The different treatment regimens were orally administered daily for a period of 17 weeks. Blood collected from the animals at the end of the test period were analyzed for erythrocyte osmotic fragility and malonaldehyde (MDA) concentration as an index of lipid peroxidation. The study showed that CPF caused significant increase in erythrocyte fragility and MDA concentration, which were ameliorated by pretreatment with vitamin C. In conclusion, the study showed that CPF-evoked erythrocyte fragility due to increased lipoperoxidative changes was ameliorated by pretreatment with vitamin C.
慢性接触毒死蜱（CPF）已被证明会导致红细胞膜的脂质过氧化变化增加。慢性 CPF 诱导的脂质过氧化变化与红细胞脆性之间的关系尚未阐明。本研究旨在评估脂质过氧化作用在 CPF 诱导的红细胞脆性中的作用以及维生素 C 的改善作用。
20 只动物随机分为四组，每组 5 只，作为本研究的对象。第 I 组大鼠作为对照组，仅给予大豆油 2 mL/kg 体重（b.w.）。第 II 组大鼠给予维生素 C（100 mg/kg b.w.），然后补充大豆油（2 mL/kg b.w.），第 III 组大鼠仅给予 CPF 10.6 mg/kg b.w.（~先前确定的半数致死剂量[LD(50)]的八分之一）。第 IV 组大鼠先用 100 mg/kg b.w.的维生素 C 预处理，30 分钟后再给予 CPF 10.6 mg/kg b.w.。不同的治疗方案每天口服，持续 17 周。在试验结束时从动物采集的血液用于分析红细胞渗透脆性和丙二醛（MDA）浓度作为脂质过氧化的指标。
研究表明 CPF 导致红细胞脆性显著增加，MDA 浓度升高，维生素 C 预处理可改善这种情况。
综上所述，本研究表明 CPF 引起的红细胞脆性增加与脂质过氧化变化有关，维生素 C 预处理可改善这种情况。
