Umosen Angela J, Ambali Suleiman F, Ayo Joseph O, Mohammed Bisala, Uchendu Chidiebere
Department of Veterinary Pharmacology, Ahmadu Bello University, Zaria, Nigeria.
Asian Pac J Trop Biomed. 2012 Aug;2(8):645-50. doi: 10.1016/S2221-1691(12)60113-0.
To evaluate the alleviating effects of melatonin on oxidative changes in the testes and pituitary gland induced by subacute chlopyrifos (CPF) exposure in rats.
Forty adult male Wistar rats divided into 4 groups of 10 animals were used for the study. Group I received soya oil (2 mL/kg) while group II was administered with melatonin (0.5 mg/kg). Group III was administered CPF only (8.5 mg/kg ∼ 1/10th of the LD50) while group IV was pretreated with melatonin (0.5 mg/kg) and then exposed to CPF (8.5 mg/kg), 10 min later. The regimens were administered by gavage once daily for a period of 28 d. At the end of the exposure period, the rats were sacrificed and the testicular tissues and pituitary glands were evaluated for the malonaldehyde (MDA) concentration and activities of superoxide dismutase (SOD) and catalase (CAT).
CPF increased MDA concentrations and reduced the activities of SOD and CAT in the testes and pituitary gland. Melatonin pretreatment reduced the testicular and pituitary MDA concentrations and improves the SOD and CAT activities.
the study showed that subacute CPF-induced oxidative stress in the testes and pituitary glands were alleviated by melatonin due to its antioxidant property.
评估褪黑素对亚急性毒死蜱（CPF）暴露诱导的大鼠睾丸和垂体氧化变化的缓解作用。
将40只成年雄性Wistar大鼠分为4组，每组10只用于研究。第一组给予大豆油（2 mL/kg），而第二组给予褪黑素（0.5 mg/kg）。第三组仅给予CPF（8.5 mg/kg ∼ LD50的1/10），而第四组先用褪黑素（0.5 mg/kg）预处理，然后在10分钟后暴露于CPF（8.5 mg/kg）。给药方案为每天经口灌胃一次，持续28天。在暴露期结束时，处死大鼠，评估睾丸组织和垂体中丙二醛（MDA）浓度以及超氧化物歧化酶（SOD）和过氧化氢酶（CAT）的活性。
CPF增加了睾丸和垂体中的MDA浓度，并降低了SOD和CAT的活性。褪黑素预处理降低了睾丸和垂体中的MDA浓度，并提高了SOD和CAT的活性。
该研究表明，由于褪黑素具有抗氧化特性，它可缓解亚急性CPF诱导的睾丸和垂体氧化应激。
