反式DSL连接酶核酶可以利用各种形式的模块来夹住其底物和酶单元。

The transDSL ligase ribozyme can utilize various forms of modules to clamp its substrate and enzyme units.

作者信息

Ishikawa Junya, Isomoto Nana, Fujita Yuki, Furuta Hiroyuki, Ikawa Yoshiya

机构信息

Department of Chemistry and Biochemistry, Graduate School of Engineering, Kyushu University, Fukuoka, Japan.

出版信息

Biosci Biotechnol Biochem. 2010;74(4):872-4. doi: 10.1271/bbb.90849. Epub 2010 Apr 7.

DOI:10.1271/bbb.90849

PMID:20378973

Abstract

TransDSL is an RNA ligase ribozyme whose enzyme unit joins two RNA fragments constituting a substrate. The enzyme unit recognizes the substrate by means of two clamp modules. We constructed active variants by replacing the original clamp module with various types of interactions. Such flexible modularity would be advantageous in the application of this ribozyme in nanobiotechnology.

摘要

转DSL是一种RNA连接酶核酶，其酶单位可连接构成底物的两个RNA片段。该酶单位通过两个钳夹模块识别底物。我们通过用各种类型的相互作用取代原始钳夹模块构建了活性变体。这种灵活的模块化在这种核酶应用于纳米生物技术方面将具有优势。