Matsumura Shigeyoshi, Ohmori Rei, Saito Hirohide, Ikawa Yoshiya, Inoue Tan
Graduate School of Biostudies, Kyoto University, Sakyo-ku, Kyoto, Japan.
FEBS Lett. 2009 Sep 3;583(17):2819-26. doi: 10.1016/j.febslet.2009.07.036. Epub 2009 Jul 23.
We previously developed a synthetic cis-acting RNA ligase ribozyme with 3'-5' joining activity termed "DSL" (designed and selected ligase). DSL was easily transformed into a trans-acting form because of its highly modular architecture. In this study, we investigated the modular properties and turnover capabilities of a trans-acting DSL, tDSL-1/GUAA. tDSL-1/GUAA exhibited remarkably high activity compared with the parental cis-acting DSL, and it attained a high turnover number. Taken together, the results indicate that a loop-receptor interaction plays a significant role in determining the activity of the trans-acting ribozyme and in its ability to perform multiple turnovers of the reaction.
我们之前开发了一种具有3'-5'连接活性的合成顺式作用RNA连接酶核酶，称为“DSL”（设计和筛选的连接酶）。由于其高度模块化的结构，DSL很容易转化为反式作用形式。在本研究中，我们研究了反式作用DSL，即tDSL-1/GUAA的模块化特性和周转能力。与亲本顺式作用DSL相比，tDSL-1/GUAA表现出显著更高的活性，并且具有高周转数。综上所述，结果表明环-受体相互作用在决定反式作用核酶的活性及其进行反应多次周转的能力方面起着重要作用。
