Nodake Yuichi, Fukumoto Shoichi, Fukasawa Masashi, Sakakibara Ryuzo, Yamasaki Nobuyuki
Department of Pharmacy, Faculty of Pharmaceutical Sciences, Nagasaki International University, Japan.
Biosci Biotechnol Biochem. 2010;74(4):721-6. doi: 10.1271/bbb.90767. Epub 2010 Apr 7.
Beta-lactoglobulin (BLG) was conjugated with the N-hydroxysuccinimide ester of the dextran-glycylglycine adduct (DG-ONSu) to reduce the immunogenicity of BLG, a major allergen of cow's milk, and some immunological properties of the conjugate (DG-BLG) were studied. The conjugate was prepared by modifying BLG with DG-ONSu and purified in a Sephadex G-100 column. The analytical data for DG-BLG indicated that 5.2 moles of DG-ONSu with a mean molecular weight of 9,300 were covalently attached to the amino groups of the BLG molecule. Conjugation with DG-ONSu greatly decreased the reactivity of BLG with anti-BLG antibodies and suppressed their production in vivo due to its shielding action for epitope(s) on the protein's molecular surface. It was also found that DG-BLG was resistant to proteolytic enzymes. These findings allow us to suggest that DG-ONSu could be advantageously used to suppress the hypersensitivity mediated by IgG antibodies in milk allergy.
将β-乳球蛋白（BLG）与葡聚糖-甘氨酰甘氨酸加合物（DG-ONSu）的N-羟基琥珀酰亚胺酯偶联，以降低牛奶主要过敏原BLG的免疫原性，并研究了偶联物（DG-BLG）的一些免疫学特性。通过用DG-ONSu修饰BLG制备偶联物，并在Sephadex G-100柱上进行纯化。DG-BLG的分析数据表明，5.2摩尔平均分子量为9300的DG-ONSu共价连接到BLG分子的氨基上。与DG-ONSu偶联大大降低了BLG与抗BLG抗体的反应性，并由于其对蛋白质分子表面表位的屏蔽作用而抑制了其在体内的产生。还发现DG-BLG对蛋白水解酶具有抗性。这些发现使我们能够提出，DG-ONSu可有利地用于抑制牛奶过敏中由IgG抗体介导的超敏反应。
