Tyczkowska K L, Voyksner R D, Aronson A L
Department of Anatomy, Physiological Sciences, and Radiology, College of Veterinary Medicine, North Carolina State University, Raleigh.
J Vet Pharmacol Ther. 1991 Mar;14(1):51-60. doi: 10.1111/j.1365-2885.1991.tb00804.x.
Metabolites of the cephapirin beta-lactam antibiotic have not previously been reported in bovine milk. The principal metabolite was tentatively identified as desacetylcephapirin by liquid chromatography with UV-VIS photodiode array (LC/UV-VIS PDA), and liquid-chromatography-mass-spectrometric (LC-MS) detection. Synthetic desacetylcephapirin was prepared by incubation of cephapirin in bovine milk and serum at 37 degrees C. Also, a method for determining cephapirin in bovine milk and serum was developed. The detection limits for cephapirin and desacetylcephapirin were estimated to be 10 and 50 micrograms/kg, respectively, for LC/UV-VIS PDA, and 100 and 500 micrograms/kg for LC-MS.
头孢匹林β-内酰胺抗生素的代谢物此前尚未在牛乳中被报道。通过带有紫外可见光电二极管阵列的液相色谱法（LC/UV-VIS PDA）以及液相色谱-质谱联用（LC-MS）检测，初步鉴定出主要代谢物为去乙酰头孢匹林。通过在37摄氏度下将头孢匹林在牛乳和血清中孵育制备出了合成去乙酰头孢匹林。此外，还开发了一种测定牛乳和血清中头孢匹林的方法。对于LC/UV-VIS PDA，头孢匹林和去乙酰头孢匹林的检测限分别估计为10微克/千克和50微克/千克，对于LC-MS则分别为100微克/千克和500微克/千克。
