变形链球菌真核型丝氨酸/苏氨酸蛋白激酶在与血链球菌种间相互作用中的作用。
Role of Streptococcus mutans eukaryotic-type serine/threonine protein kinase in interspecies interactions with Streptococcus sanguinis.
机构信息
College of Dentistry, University of Oklahoma Health Sciences Center, Oklahoma City, OK 73104, USA.
出版信息
Arch Oral Biol. 2010 May;55(5):385-90. doi: 10.1016/j.archoralbio.2010.03.012. Epub 2010 Apr 8.
OBJECTIVE
Interspecies interactions of oral streptococci involve the production and excretion of antimicrobial compounds to compete successfully during colonization. Bacteriocin production by Streptococcus mutans and hydrogen peroxide (H2O2) production by Streptococcus sanguinis have been demonstrated as crucial for the clinical relevant antagonism between both species. A potential target of H2O2 is the cell-envelop of S. mutans. In the present study, the role of cell-envelop associated eukaryotic serine/threonine protein kinase (STPK) in S. mutans during interspecies competition has been investigated.
DESIGN
Allelic replacement via homologous recombination of the STPK encoding gene with a kanamycin resistant determinant has been constructed. The mutant has been screened for the susceptibility towards cell-envelope stress. A previously developed spotting assay was used to simulate interspecies competition.
RESULTS
The STPK(-) mutant showed an increased susceptibility towards envelop stress caused by H2O2 and was significantly more inhibited during interspecies competition assays.
CONCLUSIONS
S. mutans is able to sense antimicrobial compounds excreted by competing species and can potentially adjust the cell-envelop towards an increased resistance.
目的
口腔链球菌之间的种间相互作用涉及抗菌化合物的产生和排泄，以在定植过程中成功竞争。已证明变异链球菌产生细菌素和血链球菌产生过氧化氢 (H2O2) 对于两种细菌之间的临床相关拮抗作用至关重要。H2O2 的一个潜在靶标是 S. mutans 的细胞包膜。在本研究中，研究了种间竞争过程中 S. mutans 中与细胞包膜相关的真核丝氨酸/苏氨酸蛋白激酶 (STPK) 的作用。通过同源重组用卡那霉素抗性决定因素替换 STPK 编码基因已构建。筛选突变体对细胞包膜应激的敏感性。先前开发的点样测定法用于模拟种间竞争。结果：STPK(-)突变体对 H2O2 引起的包膜应激的敏感性增加，并且在种间竞争测定中受到显著抑制。结论：S. mutans 能够感知由竞争物种分泌的抗菌化合物，并能够潜在地调整细胞包膜以增加抗性。
Zotero 插件