Post-translational modifications via serine/threonine phosphorylation and GpsB in .

Post-translational modifications (PTMs), such as protein phosphorylation, are critical regulators of bacterial physiology. Here, we present the first comprehensive phosphoproteomic analysis of , revealing extensive -phosphorylation under non-stressed conditions. Using tandem mass tag (TMT)-based mass spectrometry and phosphopeptide enrichment, we identified 231 high-confidence phosphosites on 131 proteins, representing approximately 6.7% of the detected proteome. These phosphorylated proteins were enriched in pathways related to translation, carbohydrate metabolism, and the cell cycle, suggesting a broad role for -phosphorylation in core cellular functions. To define the functional roles of the sole serine/threonine protein kinase (PknB) and phosphatase (PppL) encoded by , we analyzed phosphoproteomic and proteomic changes in Δ and Δ mutants. These mutants exhibited widespread alterations in protein abundance and phosphorylation, revealing overlapping but distinct sets of putative kinase and phosphatase substrates, including DivIVA, MapZ, MltG, and ribosomal proteins. Notably, we discovered that repression of , a predicted PknB binding partner, causes lethal defects that can be rescued by a suppressor mutation (G98R) in . This mutation restores phosphorylation of DivIVA, suggesting that GpsB regulates the PknB/PppL signaling axis to maintain appropriate phosphorylation of essential targets. This work highlights conserved and unique features of bacterial phosphosignaling and provides a foundation for future studies on PTM-mediated regulation in .