Department of Applied Science, National Hsinchu University of Education, 521 Nanda Rd, Hsinchu 30014, Taiwan.
Department of Microbiology, Kasetsart University, Bangkok 10900, Thailand.
Int J Syst Evol Microbiol. 2011 Mar;61(Pt 3):690-694. doi: 10.1099/ijs.0.020511-0. Epub 2010 Apr 9.
During surveys on yeast diversity in forest soils from Taiwan and Thailand, ten yeast strains isolated from different samples were found to have similar molecular and physiological characteristics. Sequence analysis of small subunit (SSU) rDNA, the D1/D2 domain of large subunit (LSU) rDNA and internal transcribed spacer (ITS)-5.8S rDNA demonstrated that these strains were closely related to Scheffersomyces spartinae. The novel strains could be differentiated from S. spartinae by a 0.9 % sequence divergence (5 substitutions, 0 gaps) in the D1/D2 domain of LSU rDNA, a 1.5 % divergence (8 substitutions, 0 gaps) in the ITS-5.8S rDNA and a 0.7 % divergence (12 substitutions, 2 gaps) in the SSU rDNA. The novel strains also showed specific patterns of electrophoretic karyotypes that differed from that of S. spartinae. Therefore, a novel yeast species, Candida gosingica sp. nov., is proposed to accommodate these strains. The type strain SJ7S11(T) (=BCRC 23194(T)=CBS 11433(T)) was assigned and deposited in the Bioresource Collection and Research Center (BCRC), Food Industry Development and Research Institute, Hsinchu, Taiwan, and Centraalbureau voor Schimmelcultures (CBS), Utrecht, The Netherlands.
在对来自台湾和泰国森林土壤中的酵母多样性进行调查时,从不同样本中分离出的 10 株酵母菌株被发现具有相似的分子和生理特征。小亚基(SSU)rDNA、大亚基(LSU)rDNA 的 D1/D2 结构域和内部转录间隔区(ITS)-5.8S rDNA 的序列分析表明,这些菌株与谢氏有丝孢酵母(Scheffersomyces spartinae)密切相关。这些新菌株与谢氏有丝孢酵母(S. spartinae)在 LSU rDNA 的 D1/D2 结构域中有 0.9%的序列差异(5 个取代,0 个空位),在 ITS-5.8S rDNA 中有 1.5%的差异(8 个取代,0 个空位),在 SSU rDNA 中有 0.7%的差异(12 个取代,2 个空位)。这些新菌株的电泳核型也表现出特定的模式,与谢氏有丝孢酵母(S. spartinae)不同。因此,提议建立一个新的酵母物种,即长形毕赤酵母(Candida gosingica sp. nov.),以容纳这些菌株。模式菌株 SJ7S11(T)(=BCRC 23194(T)=CBS 11433(T))被指定并保藏于食品工业发展研究所生物资源收集与研究中心(BCRC),中国台湾新竹市和荷兰乌得勒支的 Centraalbureau voor Schimmelcultures(CBS)。