sp. nov., isolated from the cocoon wall of the stag beetle .

A previous investigation of symbiotic yeasts associated with the stag beetle isolated strains of the genus from the cocoon walls, larval midgut, larval hindgut and larval tunnels. Phylogenetic analysis of the D1/D2 domains of the LSU rRNA gene sequences revealed identical sequences, indicating that they belonged to the same species, but suggested that the species was new. In this study, sequence analysis and physiological characterization identified a representative strain of these beetle-associated yeasts as a novel species in the genus . The sequence similarities of the concatenated LSU domains and internal transcribed spacer regions indicated that strain BCRC 23563 forms a well-supported and distinct species in the xylose-fermenting subclade, with the sequences for each gene differing in nt substitutions from those of previously described related species by at least 1.06% and 2.7% respectively. The physiological characteristics of the novel species were also distinct from those of the closely related described species, though it could still process xylose as is expected of stag beetle-associated symbionts. Based on the data, a novel yeast species, sp. nov., is proposed to accommodate this strain. The holotype is BCRC 23563 (ex-type strains NBRC 116731 and NCYC 4470). The MycoBank accession number is 857608.