Whitehead Institute for Biomedical Research, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA.
Genetics. 2010 Jun;185(2):513-22. doi: 10.1534/genetics.110.113944. Epub 2010 Apr 9.
Saccharomyces cerevisiae can divide asymmetrically so that the mother and daughter cells have different fates. We show that the RNA-binding protein Khd1 regulates asymmetric expression of FLO11 to determine daughter cell fate during filamentous growth. Khd1 represses transcription of FLO11 indirectly through its regulation of ASH1 mRNA. Khd1 also represses FLO11 through a post-transcriptional mechanism independent of ASH1. Cross-linking immunoprecipitation (CLIP) coupled with high-throughput sequencing shows that Khd1 directly binds repetitive sequences in FLO11 mRNA. Khd1 inhibits translation through this interaction, establishing feed-forward repression of FLO11. This regulation enables changes in FLO11 expression between mother and daughter cells, which establishes the asymmetry required for the developmental transition between yeast form and filamentous growth.
酿酒酵母可以不对称分裂,使得母细胞和子细胞具有不同的命运。我们表明,RNA 结合蛋白 Khd1 通过调节 FLO11 的不对称表达来决定丝状生长过程中子细胞的命运。Khd1 通过其对 ASH1 mRNA 的调节间接抑制 FLO11 的转录。Khd1 还通过独立于 ASH1 的转录后机制抑制 FLO11。交联免疫沉淀 (CLIP) 与高通量测序相结合表明,Khd1 直接结合 FLO11 mRNA 中的重复序列。Khd1 通过这种相互作用抑制翻译,从而建立 FLO11 的前馈抑制。这种调节使得母细胞和子细胞之间的 FLO11 表达发生变化,从而为酵母形态和丝状生长之间的发育转变建立所需的不对称性。