Cromie Gareth A, Tan Zhihao, Hays Michelle, Sirr Amy, Jeffery Eric W, Dudley Aimée M
Pacific Northwest Research Institute, Seattle, Washington 98122.
Institute of Medical Biology, Agency for Science, Technology and Research, Singapore 138648.
G3 (Bethesda). 2017 Aug 7;7(8):2845-2854. doi: 10.1534/g3.117.042440.
Biofilm formation by microorganisms is a major cause of recurring infections and removal of biofilms has proven to be extremely difficult given their inherent drug resistance . Understanding the biological processes that underlie biofilm formation is thus extremely important and could lead to the development of more effective drug therapies, resulting in better infection outcomes. Using the yeast as a biofilm model, overexpression screens identified , , , , , and as regulators of biofilm formation. Subsequent RNA-seq analysis of biofilm and nonbiofilm-forming strains revealed that all of the overexpression strains, other than and , were adopting a single differential expression profile, although induced to varying degrees. adopted a separate profile, while the expression profile of reflected the common pattern seen in most of the strains, plus substantial -specific expression changes. We interpret the existence of the common transcriptional pattern seen across multiple, unrelated overexpression strains as reflecting a transcriptional state, that the yeast cell can access through regulatory signaling mechanisms, allowing an adaptive morphological change between biofilm-forming and nonbiofilm states.
微生物形成生物膜是反复感染的主要原因，鉴于生物膜固有的耐药性，事实证明清除生物膜极其困难。因此，了解生物膜形成背后的生物学过程极为重要，这可能会带来更有效的药物治疗方法，从而改善感染治疗效果。以酵母作为生物膜模型，通过过表达筛选确定了……、……、……、……、……和……作为生物膜形成的调节因子。随后对形成生物膜和不形成生物膜的菌株进行RNA测序分析发现，除了……和……之外，所有过表达菌株都呈现出单一的差异表达谱，尽管诱导程度有所不同。……呈现出单独的谱，而……的表达谱反映了大多数菌株中常见的模式，以及大量特定于……的表达变化。我们将多个不相关的过表达菌株中出现的共同转录模式解释为反映了一种转录状态，酵母细胞可以通过调节信号机制进入这种状态，从而在生物膜形成状态和非生物膜状态之间实现适应性形态变化。
