γ-谷氨酰转肽酶活力大肠杆菌催化谷氨酸γ-甲酯和乙胺合成茶氨酸。
Synthesis of theanine from glutamic acid gamma-methyl ester and ethylamine catalyzed by Escherichia coli having gamma-glutamyltranspeptidase activity.
机构信息
State Key Laboratory of Pharmaceutical Biotechnology, School of Life Science, Nanjing University, Nanjing 210093, People's Republic of China.
出版信息
Biotechnol Lett. 2010 Aug;32(8):1147-50. doi: 10.1007/s10529-010-0273-1. Epub 2010 Apr 11.
Glutamic acid gamma-methyl ester (GAME) was used as substrate for theanine synthesis catalyzed by Escherichia coli cells possessing gamma-glutamyltranspeptidase activity. The yield was about 1.2-fold higher than with glutamine as substrate. The reaction was optimal at pH 10 and 45 degrees C, and the optimal substrate ratio of GAME to ethylamine was 1:10 (mol/mol). With GAME at 100 mmol, 95 mmol theanine was obtained after 8 h.
谷氨酸γ-甲酯（GAME）被用作具有谷氨酰转肽酶活性的大肠杆菌细胞合成茶氨酸的底物。与谷氨酰胺作为底物相比，收率约提高了 1.2 倍。反应的最佳 pH 值为 10，最佳温度为 45°C，GAME 与乙胺的最佳底物比为 1:10（摩尔/摩尔）。当 GAME 为 100mmol 时，8 小时后可得到 95mmol 茶氨酸。
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