Division of Applied Biology, Graduate School of Science and Technology, Kyoto Institute of Technology, Matsugasaki, Sakyo-ku, Kyoto, 606-8585, Japan.
Biotechnol Lett. 2010 Dec;32(12):1877-81. doi: 10.1007/s10529-010-0364-z. Epub 2010 Jul 31.
The optimal reaction conditions for the synthesis of γ-glutamylglutamine using γ-glutamyltranspeptidase from Escherichia coli were determined. The maximum yield of γ-glutamylglutamine (110 mM) was obtained using 250 mM L: -glutamine and 1.1 U γ-glutamyltranspeptidase/ml at pH 10.5 and at 37°C for 7 h; the conversion of glutamine to γ-glutamylglutamine was 88%.
确定了利用大肠杆菌谷氨酰转肽酶合成γ-谷氨酰-γ-谷氨酰胺的最佳反应条件。在 pH 10.5 和 37°C 下反应 7 小时，使用 250 mM L: -谷氨酰胺和 1.1 U/ml 谷氨酰转肽酶，可获得最大产量的 γ-谷氨酰-γ-谷氨酰胺（110 mM），谷氨酰胺转化为 γ-谷氨酰-γ-谷氨酰胺的转化率为 88%。
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