Department of Internal Medicine, Albert Einstein College of Medicine, Bronx, NY, USA.
Immunol Lett. 2010 Jul 8;131(2):151-8. doi: 10.1016/j.imlet.2010.04.001. Epub 2010 Apr 10.
The active form of vitamin D, 1alpha,25-dihydroxyvitamin D(3) [1alpha,25(OH)(2)D(3)], has been reported to influence the functioning of the immune system by targeting the activities of cellular signaling pathways, in addition to its direct genomic effects. One of the signaling pathways reported to be targeted by vitamin D is the NF-kappaB pathway, which is highly active in most immune cell types, including T cells. However, the effects of vitamin D on the NF-kappaB pathway in T cells are not fully understood. Therefore, we examined the effects of 1alpha,25(OH)(2)D(3) on the NF-kappaB pathway in the Jurkat cell line, a human T cell line that constitutively expresses endogenous vitamin D receptor. We found that 1alpha,25(OH)(2)D(3) does not inhibit the induction of IkappaBalpha degradation and the expression of an NF-kappaB-dependent reporter gene in Jurkat cells following treatment with PMA/ionomycin. Also, 1alpha,25(OH)(2)D(3) did not suppress the activation of NF-kappaB by TNFalpha or PHA. Furthermore, we demonstrate that 1alpha,25(OH)(2)D(3) does not block the induction of CD69, which is an NF-kappaB target gene and an early T cell activation marker. Therefore, we conclude that vitamin D does not modulate the activity of the NF-kappaB pathway in Jurkat cells.
活性维生素 D 形式，1α，25-二羟维生素 D(3)[1α，25(OH)2D(3)]，据报道通过靶向细胞信号通路的活性来影响免疫系统的功能，除了其直接的基因组效应。据报道，维生素 D 靶向的信号通路之一是 NF-κB 通路，该通路在大多数免疫细胞类型中都高度活跃，包括 T 细胞。然而，维生素 D 对 T 细胞中 NF-κB 通路的影响尚未完全阐明。因此，我们研究了 1α，25(OH)2D(3)对 Jurkat 细胞系（一种表达内源性维生素 D 受体的人 T 细胞系）中 NF-κB 通路的影响。我们发现，1α，25(OH)2D(3)在 PMA/离子霉素处理后不会抑制 Jurkat 细胞中 IkappaBalpha 降解的诱导和 NF-κB 依赖性报告基因的表达。此外，1α，25(OH)2D(3)也不会抑制 TNFα或 PHA 激活 NF-κB。此外，我们证明 1α，25(OH)2D(3)不会阻断 NF-κB 靶基因和早期 T 细胞激活标志物 CD69 的诱导。因此，我们得出结论，维生素 D 不会调节 Jurkat 细胞中 NF-κB 通路的活性。
