Laboratoire de Chimie des Substances Naturelles, Faculté des Sciences et Techniques, Limoges, France.
Bioresour Technol. 2010 Aug;101(16):6538-44. doi: 10.1016/j.biortech.2010.03.054. Epub 2010 Apr 10.
Hardwood 4-O-methylglucuronoxylans (MGX) are classically isolated by a delignification step, using a sodium chlorite solution, followed by alkaline extraction, an efficient, although potentially polluting process. In this work, we propose a chlorine-free environmentally-friendly process for MGX extraction from Castanea sativa Mill. Chestnut sawdust was first delignified using metalled phthalocyanine or porphyrin in presence of hydrogen peroxide. Then, MGX were easily extracted by hot water. This protocol, repeated with different incubation times and temperatures, led to the selective extraction of acetylated MGX with decent yields reaching 12%. The best results were obtained after delignification using iron tetrapyrrole macrocycles. However, lower degrees of polymerization of MGX were observed in comparison to the classical method, suggesting that this new process affects the structures of xylans.
硬木 4-O-甲基葡萄糖醛酸木聚糖 (MGX) 通常通过使用亚氯酸钠溶液进行脱木质素步骤来分离，然后进行碱性提取，这是一种有效的方法，但也有潜在的污染问题。在这项工作中，我们提出了一种从栗木 (Castanea sativa Mill.) 中提取 MGX 的无氯环保工艺。栗木屑首先使用金属酞菁或卟啉在过氧化氢存在下进行脱木质素处理。然后，MGX 很容易通过热水提取。该方案通过不同的孵育时间和温度重复进行，导致乙酰化 MGX 的选择性提取，收率达到 12%。使用四吡咯铁大环化合物进行脱木质素后，得到的结果最好。然而，与经典方法相比，MGX 的聚合度较低，这表明这个新过程会影响木聚糖的结构。
