VTT Technical Research Centre of Finland, P.O.Box 1300, FI-33101, Tampere, Finland.
J Fluoresc. 2010 Sep;20(5):1003-8. doi: 10.1007/s10895-010-0647-8. Epub 2010 Apr 13.
A surface-sensitive fluorescence measurement platform is utilised in the detection of morphine. The platform is based on a polystyrene parabolic lens that enables the simultaneous application of total internal reflection excitation and supercritical angle fluorescence detection in the measurements. The molecular recognition of morphine is based on two antibodies, one against morphine and the other against the immune complex formed between the anti-morphine antibody and a morphine molecule. The antibodies are applied in a sandwich-like format in a one-step test, where the molecular binding onto a liquid-solid-interface is monitored in real time. Morphine concentrations between 0.6 and 18.2 ng/mL were reliably determined in 60 s, while concentrations down to 2.7 ng/mL were detected already in 20 s. With appropriate recognition molecules the technique is applicable also to other drugs and small analytes.
利用一种对表面敏感的荧光测量平台来检测吗啡。该平台基于一个聚苯乙烯抛物线透镜，可在测量中同时应用全内反射激发和超临界角荧光检测。吗啡的分子识别基于两种抗体，一种针对吗啡，另一种针对抗吗啡抗体与吗啡分子形成的免疫复合物。抗体以三明治样的形式应用于一步测试中，实时监测分子在液-固界面上的结合情况。在 60 秒内可靠地确定了 0.6 至 18.2 ng/mL 之间的吗啡浓度，而在 20 秒内已经检测到低至 2.7 ng/mL 的浓度。通过适当的识别分子，该技术也可应用于其他药物和小分子分析物。
