Physikalisch-Chemisches Institut, Universität Zürich, Winterthurerstrasse 190, CH-8057 Zürich, Switzerland; E-Mails:
Biosensors (Basel). 2013 Feb 8;3(1):108-15. doi: 10.3390/bios3010108. eCollection 2013 Mar.
We present an immunoassay for Interferon-γ (IFN-γ) with a limit of detection of 1.9 pM (30 pg/mL) and a linear concentration range spanning three orders of magnitude. The developed one-step assay takes only 12 min and can replace the time-consuming and labor-intensive enzyme-linked immunosorbent assay (ELISA). The solid-phase sandwich assay is performed on a new measurement system comprising single-use test tubes and a compact fluorescence reader. The polymer tubes contain an optical configuration for the detection of supercritical angle fluorescence, allowing for highly sensitive real-time binding measurements.
我们提出了一种检测干扰素-γ（IFN-γ）的免疫分析方法，其检测限为 1.9 pM（30 pg/mL），线性浓度范围跨越三个数量级。开发的一步法检测仅需 12 分钟，可替代费时费力的酶联免疫吸附测定（ELISA）。固相夹心检测在一个新的测量系统上进行，该系统包括一次性使用的试管和紧凑型荧光读取器。聚合物试管包含用于检测超临界角荧光的光学结构，允许进行高灵敏度的实时结合测量。
