RNA转染细胞微阵列的功能研究。

Functional studies on RNA-transfected cell microarrays.

作者信息

Fjeldbo Christina Saeten, Misund Kristine, Günther Clara-Cecilie, Langaas Mette, Steigedal Tonje Strømmen, Thommesen Liv, Laegreid Astrid, Bruland Torunn

机构信息

Department of Cancer Research and Molecular Medicine, Norwegian University of Science and Technology (NTNU), Trondheim, Norway.

出版信息

Methods Mol Biol. 2010;629:175-91. doi: 10.1007/978-1-60761-657-3_12.

DOI:10.1007/978-1-60761-657-3_12

PMID:20387150

Abstract

RNA-transfected cell microarray shows great promise in functional genomics. By printing siRNA complexed with transfection reagent on glass slides, arrays of transfected cells are formed in which the phenotypic consequences of gene suppression can be investigated. Using reporter plasmids with fluorescence intensity as output data, we have developed a strategy for statistical analysis of the intensity data from medium-scale functional studies using data from several experimental replicates.

摘要

RNA转染细胞微阵列在功能基因组学中显示出巨大的前景。通过将与转染试剂复合的小干扰RNA（siRNA）打印在载玻片上，形成转染细胞阵列，在其中可以研究基因抑制的表型后果。利用以荧光强度作为输出数据的报告质粒，我们开发了一种统计分析策略，用于对来自多个实验复制品的中规模功能研究强度数据进行分析。