[磷-31磁共振波谱检测禁食家兔肝脏中的生化代谢变化]

Xu Xiu-fang, Yu Ri-sheng, Liu Rui, Sun Jian-zhong, Chen Yi-hong, Chen Jian, Zhang Min-ming

Department of Radiology, Zhejiang Medical College, Hangzhou 310053, China.

Zhejiang Da Xue Xue Bao Yi Xue Ban. 2010 Mar;39(2):143-9. doi: 10.3785/j.issn.1008-9292.2010.02.006.

OBJECTIVE

To investigate the biochemical metabolic changes detected by phosphorus-31 MR spectroscopy ((31)P MRS) with pathologic changes in the liver of fasting rabbits.

METHODS

A total of 22 rabbits were under the starvation up to death to establish animal models. Hepatic (31)P MRS was performed in different period of 10 rabbits including normal condition, over-starvation, agonal condition and death after 30 min. Other 9 rabbits were divided into three type including over-starvation, agonal condition and death group with 3 rabbits in each group, and 3 healthy rabbits served as controls. All the 12 rabbits were sacrificed for the hepatic pathological examination. The MR examination was performed on a 1.5 T imager using a 1H/31P surface coil by the 2D chemical shift imaging technique. The relative quantities of phosphomonoesters (PME), phosphodiesters (PDE), inorganic phosphate (Pi) and adenosine triphosphate (ATP) were measured.

RESULTS

All the relative quantification of phosphorus metabolites were changed significantly from starvation to death (X(2)=23.13-35.41, P<0.01). The relative quantifications of ATP of normal condition, over-starvation, agonal condition and death were 2.54 +/-0.53, 1.73 +/-0.14, 0.88 +/-0.23 and 0.05 +/-0.08, respectively (rs=1.0, P<0.01). The relative quantifications of PDE from normal to death were 1.25 +/-0.54, 2.76 +/-0.23, 3.33 +/-0.49 and 3.87 +/-0.43, respectively, and those of Pi were 0.42 +/-0.02, 0.65 +/-0.05, 0.89 +/-0.15 and 0.99 +/-0.08, respectively (rs=1.0, P <0.01). The relative quantifications of PME were also significantly changed (rs=0.4, P=0.6). The pathologic changes of normal condition, over-starvation, agonal condition and death: decreased size of hepatocytes, loss of cell number, cellular swelling, degeneration and cell necrosis or hepatic hemorrhage became more and more pronounced.

CONCLUSION

(31)P MRS can monitor dynamic changes of relative quantification of phosphorus metabolites, which are correlated with the pathological severity of acute hepatic injury by fasting.

目的

研究磷-31磁共振波谱（³¹P MRS）检测的生化代谢变化与禁食家兔肝脏病理变化的关系。

方法

选取22只家兔，禁食直至死亡以建立动物模型。对10只家兔在不同时期进行肝脏³¹P MRS检查，包括正常状态、过度饥饿、濒死状态及死亡后30分钟。另外9只家兔分为过度饥饿、濒死状态和死亡组，每组3只，3只健康家兔作为对照。所有12只家兔均处死后进行肝脏病理检查。MR检查在1.5T成像仪上采用1H/³¹P表面线圈通过二维化学位移成像技术进行。测量磷酸单酯（PME）、磷酸二酯（PDE）、无机磷酸（Pi）和三磷酸腺苷（ATP）的相对含量。

结果

从饥饿到死亡，所有磷代谢产物的相对定量均有显著变化（X²=23.13 - 35.41，P<0.01）。正常状态、过度饥饿、濒死状态和死亡时ATP的相对定量分别为2.54±0.53、1.73±0.14、0.88±0.23和0.05±0.08（rs=1.0，P<0.01）。从正常到死亡，PDE的相对定量分别为1.25±0.54、2.76±0.23、3.33±0.49和3.87±0.43，Pi的相对定量分别为0.42±0.02、0.65±0.05、0.89±0.15和0.99±0.08（rs=1.0，P<0.01）。PME的相对定量也有显著变化（rs=0.4，P=0.6）。正常状态、过度饥饿、濒死状态和死亡时的病理变化：肝细胞体积减小、细胞数量减少、细胞肿胀、变性以及细胞坏死或肝出血越来越明显。