Song Shu-jing, Feng Xin, Guo Jing-jing, Iu Ya-nan, Lun Wen-hui, Wei Hong-shan, Liu Shun-ai
Institute of Infectious Diseases, Capital Medical University, China
Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi. 2009 Oct;23(5):361-3.
Analyzing the relationships between peripheral blood CD4+ CD25hi regulatory T (Treg) cells and peripheral blood immune status or plasma HIV-lviral load in HIV-infected individuals,so as to determine whether Treg were related to the progression of HIV-infected disease.
116 HIV-infected patients in different stages and 21 healthy control individuals were included in this study. The CD4+ and CD8+ T cell counts were determined by a standard 4-color flow cytometry technique. The Treg cells were examined with 3-color immune staining flow cytometry. The plasma HIV-1 viral load was detected by real time PCR.
The frequencies of Treg cells decreased in HIV-infected individuals with high CD4+ T cell counts( > 300/microl) compared with normal controls. With the progression of disease the frequencies of Treg cells were raised gradually, until were increased in HIV-infected individuals with low levels of CD4+ T cell counts ( < 100/microl). In addition, the frequencies of Treg cells were inversely related to CD4+ T cell counts and CD4+ /CD8+ ratio, data showed a statistically significant (respectively, r = -0.564, P < 0.001; r = -0.377, P < 0.001). Furthermore, the proportions of Treg cells were closely related to plasma HIV-1 RNA viral load (r = 0.514, P < 0.001).
CD4 CD25hi Treg cells should be a kind of important cells participating the immunopathogenesis of AIDS. It may play different roles in different stages of HIV-infected disease. The exact mechanism of Treg cells in the progression of the HIV-infected disease needs to be investigate further.
分析HIV感染者外周血CD4+CD25hi调节性T细胞（Treg）与外周血免疫状态或血浆HIV-1病毒载量之间的关系，以确定Treg是否与HIV感染疾病的进展有关。
本研究纳入116例不同阶段的HIV感染患者和21名健康对照者。采用标准四色流式细胞术检测CD4+和CD8+T细胞计数。用三色免疫染色流式细胞术检测Treg细胞。通过实时PCR检测血浆HIV-1病毒载量。
与正常对照相比，CD4+T细胞计数高（>300/μl）的HIV感染者中Treg细胞频率降低。随着疾病进展，Treg细胞频率逐渐升高，直至在CD4+T细胞计数低（<100/μl）的HIV感染者中增加。此外，Treg细胞频率与CD4+T细胞计数和CD4+/CD8+比值呈负相关，数据显示具有统计学意义（分别为r=-0.564，P<0.001；r=-0.377，P<0.001）。此外，Treg细胞比例与血浆HIV-1RNA病毒载量密切相关（r=0.514，P<0.001）。
CD4 CD25hi Treg细胞应是参与艾滋病免疫发病机制的一种重要细胞。它可能在HIV感染疾病的不同阶段发挥不同作用。Treg细胞在HIV感染疾病进展中的确切机制有待进一步研究。
