Zhang Han, Xiao He, Wang Da-jiang, Zhang Lei, Wang Li-qiang, Li Yan, Huang Yi-fei
Ophthalmic Center of PLA, Department of Ophthalmology, General Hospital of PLA, Beijing 100853, China.
Zhonghua Yan Ke Za Zhi. 2010 Jan;46(1):51-5.
To discuss the mechanism of J2 on preventing mice's corneal rejection.
In experimental study, Balb/c mice had been divided into A, B, C, D, E groups randomly. There were 13 mice in each of Group A, C and E, while 7 in both Group B and D. Group A did no management; Group B, autograft control; Group C, D and E, allograft groups (C57BL/6 were used as donors); Group B and Group C were given placebo; Group D and Group E were treated with orally cyclosporine A (CsA) (10 mg per kilogram of body weight) and J2 (15 mg per kilogram of body weight), respectively. To observe the variation of lymphocyte subgroup of peripheral blood mononuclear cells in different groups by flow cytometer analysis every week after corneal transplantation. DTH assay had been done at week 3, cytokines including interleukin-2 (IL-2), IL-10, interferon-gamma (IFN-gamma), secreting level of mouse spleen cells detected by ELLISPOT assay on day 18 after corneal transplantation. To apply single factor analysis of variance, single factor analysis of variance for repeat measured data and so on to analyze the data.
The results of flow cytometer analysis showed: lymphocyte subgroup of peripheral blood mononuclear cells had not proliferated specifically in Group E, compared to Group B and Group D (for CD3(+)CD4(+)T lymphocyte, E-B, P = 0.776, E-D, P = 0.606; for CD3(+)CD8(+)T lymphocyte, E-B, P = 0.941, E-D, P = 0.482). DTH assay showed low reaction to both donor's and third strain mouse spleen cells in Group E (F = 1.00, P = 0.422). The results of ELLISPOT assay indicated: the spot level of IL-2, IFN-gamma in Group E raised slightly compared to Group A, but compared to Group B, there was no statistically significant difference (for IL-2, P = 0.832;for IFN-gamma, P = 0.356). The spot level of IL-10 between all groups had no statistically significant difference (F = 2.911, P = 0.240).
J2 could block up the process of antigen presentation by inhibiting CD4/major histocompatibility complex-II (MHC-II) moleculeonania, while secreting IL-2, IL-10 and IFN-gamma did not have specificity change.
探讨J2预防小鼠角膜移植排斥反应的机制。
实验研究中，将Balb/c小鼠随机分为A、B、C、D、E组。A组、C组和E组每组13只小鼠，B组和D组每组7只小鼠。A组不做处理；B组为自体移植对照组；C组、D组和E组为同种异体移植组（供体为C57BL/6小鼠）；B组和C组给予安慰剂；D组和E组分别口服环孢素A（CsA）（每千克体重10毫克）和J2（每千克体重15毫克）。角膜移植后每周通过流式细胞仪分析观察不同组外周血单个核细胞淋巴细胞亚群的变化。在第3周进行迟发型超敏反应（DTH）试验，在角膜移植后第18天通过酶联免疫斑点分析（ELLISPOT assay）检测小鼠脾细胞分泌的细胞因子，包括白细胞介素-2（IL-2）、IL-10、干扰素-γ（IFN-γ）。应用单因素方差分析、重复测量数据的单因素方差分析等对数据进行分析。
流式细胞仪分析结果显示：与B组和D组相比，E组外周血单个核细胞淋巴细胞亚群未出现特异性增殖（对于CD3(+)CD4(+)T淋巴细胞，E-B，P = 0.776，E-D，P = 0.606；对于CD3(+)CD8(+)T淋巴细胞，E-B，P = 0.941，E-D，P = 0.482）。DTH试验显示E组对供体和第三品系小鼠脾细胞的反应均较低（F = 1.00，P = 0.422）。ELLISPOT分析结果表明：与A组相比，E组IL-2、IFN-γ的斑点水平略有升高，但与B组相比，差异无统计学意义（对于IL-2，P = 0.832；对于IFN-γ，P = 0.356）。各组间IL-10的斑点水平差异无统计学意义（F = 2.911，P = 0.240）。
J2可通过抑制CD4/主要组织相容性复合体-II（MHC-II）分子的抗原呈递过程，而分泌IL-2、IL-10和IFN-γ未发生特异性改变。
