Zhang Han, Huang Yi-fei, Wang Li-qiang, Liu Li
Department of Ophthalmology, General Hospital of PLA, Beijing 100853, China.
Zhonghua Yan Ke Za Zhi. 2007 Jul;43(7):608-12.
To investigate the effects of small non-peptidic CD4 inhibitor J2 on the prevention of allograft rejection in mouse corneal transplantation.
Twenty-three C57BL/6 mice (donor) and 76 BALB/c mice (recipients) were used to establish corneal allograft model, and were divided into 4 groups randomly. Group A, autograft control; Group B, allograft control (placebo was given in both control groups); Group C and Group D, allograft groups, were treated with orally ciclosporin A and J2, respectively. Drugs were delivered for 12 days beginning on the day of transplantation. Graft survival was assessed by Kaplan-Meier analysis. On day 21 after transplantation, flow cytometry analysis of peripheral blood mononuclear cells and histological examination of graft were performed.
No graft rejection was observed in Group A. The average transplant survival time of Group B was (17.8 +/- 2.1) d. In Group D, the transplant survival time showed a statistically prolongation to (40.6 +/- 8.3) d (P = 0.00) as compared with Group B, but the survival time was less than that of Group A (P = 0.04). There was no statistically significant difference between Group D and Group C (38.1 +/- 9.9) d (P = 0.99). The results of flow cytometry analysis showed that CD4(+) cells and CD8(+) cells in peripheral blood mononuclear cells did not increase in Group D. Fewer inflammatory cells were found in the corneal allograft in Group D.
J2 could inhibit rejection and prolong corneal allograft survival time in mouse.
探讨小分子非肽类CD4抑制剂J2对小鼠角膜移植中同种异体移植排斥反应的预防作用。
采用23只C57BL/6小鼠（供体）和76只BALB/c小鼠（受体）建立角膜同种异体移植模型，并随机分为4组。A组为自体移植对照组；B组为同种异体移植对照组（两组对照组均给予安慰剂）；C组和D组为同种异体移植组，分别口服环孢素A和J2进行治疗。从移植当天开始给药12天。通过Kaplan-Meier分析评估移植物存活情况。移植后第21天，对外周血单个核细胞进行流式细胞术分析，并对移植物进行组织学检查。
A组未观察到移植物排斥反应。B组平均移植存活时间为（17.8±2.1）天。与B组相比，D组移植存活时间在统计学上延长至（40.6±8.3）天（P = 0.00），但存活时间短于A组（P = 0.04）。D组与C组（38.1±9.9）天之间无统计学显著差异（P = 0.99）。流式细胞术分析结果显示，D组外周血单个核细胞中的CD4(+)细胞和CD8(+)细胞未增加。D组角膜同种异体移植中发现的炎性细胞较少。
J2可抑制小鼠角膜移植排斥反应并延长移植物存活时间。
