Shaw Michael, Hall Simon, Knox Steven, Stevens Richard, Paterson Carl
National Physical Laboratory, Hampton Road, Teddington, Middlesex, TW11 0LW, UK.
Opt Express. 2010 Mar 29;18(7):6900-13. doi: 10.1364/OE.18.006900.
In this paper we describe the wavefront aberrations that arise when imaging biological specimens using an optical sectioning microscope and generate simulated wavefronts for a planar refractive index mismatch. We then investigate the capability of two deformable mirrors for correcting spherical aberration at different focusing depths for three different microscope objective lenses. Along with measurement and analysis of the mirror influence functions we determine the optimum mirror pupil size and number of spatial modes included in the wavefront expansion and we present measurements of actuator linearity and hysteresis. We find that both mirrors are capable of correcting the wavefront aberration to improve imaging and greatly extend the depth at which diffraction limited imaging is possible.
在本文中，我们描述了使用光学切片显微镜对生物样本成像时出现的波前像差，并针对平面折射率失配生成了模拟波前。然后，我们研究了两个可变形镜针对三种不同显微镜物镜在不同聚焦深度校正球差的能力。除了测量和分析镜影响函数外，我们还确定了波前展开中包含的最佳镜光瞳尺寸和空间模式数量，并给出了致动器线性度和滞后的测量结果。我们发现，这两个镜都能够校正波前像差以改善成像，并大大扩展实现衍射极限成像的深度。
