高效液相色谱法测定毛叶锡生藤和中华锡生藤中黄连素的含量。

HPLC Estimation of berberine in Tinospora cordifolia and Tinospora sinensis.

作者信息

Srinivasan G V, Unnikrishnan K P, Rema Shree A B, Balachandran Indira

机构信息

Phytochemistry Division, Centre for Medicinal Plants Research, Arya Vaidya Sala, Changuvetty, Kottakkal - 676 503, Kerala, India.

出版信息

Indian J Pharm Sci. 2008 Jan;70(1):96-9. doi: 10.4103/0250-474X.40341.

DOI:10.4103/0250-474X.40341

PMID:20390090

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2852071/

Abstract

A high-performance liquid chromatographic method for the estimation of berberine in the stem of Tinospora cordifolia (Willd.) Miers. ex Hook.f. and Thoms. and Tinospora sinensis (Lour.) Merrill is described. The dried stems of T. cordifolia and T. sinensis were defatted with petroleum ether (60-80 degrees ). The marc was dried and further extracted with methanol. The concentration of berberine in methanol extract was determined using a C-18 reverse phase column with a mobile phase of acetonitrile:water (10:90 v/v) at a flow rate of 0.6 ml/min and with UV detection at 266 nm. TLC and HPLC comparison of both the species revealed significant variation in the chemical constitution of the two species. This observation becomes important in the context of the use of T. sinensis in place of the genuine drug T. cordifolia.

摘要

描述了一种用于测定心叶青牛胆（Willd.）Miers. ex Hook.f. and Thoms. 以及中华青牛胆（Lour.）Merrill茎中黄连素含量的高效液相色谱法。心叶青牛胆和中华青牛胆的干燥茎用石油醚（60 - 80度）脱脂。药渣干燥后再用甲醇提取。使用C - 18反相柱，以乙腈:水（10:90 v/v）为流动相，流速为0.6 ml/min，在266 nm处进行紫外检测，测定甲醇提取物中黄连素的浓度。两种植物的薄层色谱（TLC）和高效液相色谱（HPLC）比较显示，这两个物种的化学成分存在显著差异。在使用中华青牛胆替代正品药材心叶青牛胆的背景下，这一观察结果具有重要意义。