Division of Genomic Resources, ICAR-National Bureau of Plant Genetic Resources, New Delhi 110012, India.
Department of Biotechnology, School of Engineering and Technology, Sharda University, Greater Noida 201306, India.
Genes (Basel). 2022 Nov 5;13(11):2042. doi: 10.3390/genes13112042.
In the present study, novel genomic-SSR (g-SSR) markers generated in our laboratory were used to characterize and related species. The g-SSR marker was also compared with EST-SSR and SCoT markers used earlier in our laboratory to assess the genetic diversity of . A total of 26 accessions of and 1 accession each of and were characterized using 65 novel g-SSR markers. A total of 125 alleles were detected with 49 polymorphic g-SSR markers. The number of alleles per locus varied from 1-4 with a mean value of 2.55 alleles per locus. Mean PIC, gene diversity, and heterozygosity were estimated to be 0.33, 0.41, and 0.65, respectively. The two species, namely and , showed cross-species transferability of g-SSRs developed in . The success rate of cross-species transferability in was 95.3% and 93.8% in , proving the usefulness of this marker in genetic diversity studies of related species. The accessions were also used for molecular characterization using SCoT and EST-SSR markers and compared for genetic diversity and cross-species transferability. The PIC, gene diversity, heterozygosity, and principal coordinate analysis showed that g-SSR is the better maker for a genetic diversity study of Additionally, high cross-species transferability of g-SSRs was found (95.3% and 93.8%) compared to EST-SSRs (68.8% and 67.7%) in and , respectively.
在本研究中，我们实验室生成的新型基因组 SSR（g-SSR）标记被用于表征 和相关物种。该 g-SSR 标记还与我们实验室之前使用的 EST-SSR 和 SCoT 标记进行了比较，以评估 的遗传多样性。总共使用 65 个新的 g-SSR 标记对 26 个 和 1 个 、 各 1 个的品系进行了表征。用 49 个多态性 g-SSR 标记共检测到 125 个等位基因。每个位点的等位基因数从 1-4 不等，平均每个位点 2.55 个等位基因。平均 PIC、基因多样性和杂合度分别估计为 0.33、0.41 和 0.65。这两个物种，即 和 ，显示了 在 中开发的 g-SSR 的跨物种可转移性。 在 中的跨物种可转移性成功率为 95.3%，在 中为 93.8%，证明了该标记在相关物种遗传多样性研究中的有用性。还使用 SCoT 和 EST-SSR 标记对 品系进行了分子表征，并比较了遗传多样性和跨物种可转移性。PIC、基因多样性、杂合度和主坐标分析表明，g-SSR 是研究 遗传多样性的更好标记。此外，与 EST-SSR（分别为 68.8%和 67.7%）相比，g-SSR 在 和 中的跨物种可转移性较高（分别为 95.3%和 93.8%）。
