Division of EcoScience, Ewha Womans University, Seoul, Korea.
J Environ Sci Health A Tox Hazard Subst Environ Eng. 2010;45(3):389-94. doi: 10.1080/10934520903467832.
In this study, the heavy metal uptake ability of two plant species, barnyard grass and Indian mallow, and the effects of associated micro-communities on the rhizosphere of these plants were investigated in metal-contaminated sites. In addition, the effectiveness of phytoremediation using these plants was compared under field and pot conditions. To accomplish this analysis, phytoremediation of general military shooting range soil was conducted for 8 weeks under the two conditions. The results showed that metal uptake by plants and reductions in soil metal concentration were lower in the field than in pots. However, soil dehydrogenase activities and microbial diversity increased in response to phytoremediation in the field. Specifically, the soil dehydrogenase activities of barnyard grass in field soils were 3-fold higher than those of potted soils. Moreover, the denaturing gradient gel electrophoresis patterns revealed that groups formed according to plant species. Finally, the Shannon-Weaver diversity index and Simpson dominance index were higher in the rhizosphere of barnyard grass than in the rhizosphere of Indian mallow under field conditions. These results indicate that it is difficult to apply the results obtained from pot experiments to field conditions. These findings can be used to inform future studies conducted to determine if field sites are suitable for phytoremediation based on the results of pot studies.
在这项研究中，研究了两种植物物种——稗草和野西瓜苗——在受金属污染的地点对重金属的吸收能力，以及相关微生物群落对这些植物根际的影响。此外，还比较了在田间和盆栽条件下使用这些植物进行植物修复的效果。为了完成这项分析，在这两种条件下，对一般军用射击场土壤进行了为期 8 周的植物修复。结果表明，与盆栽相比，田间条件下植物对金属的吸收和土壤中金属浓度的降低较低。然而，土壤脱氢酶活性和微生物多样性随着田间植物修复而增加。具体来说，稗草根际土壤的脱氢酶活性比盆栽土壤高 3 倍。此外，变性梯度凝胶电泳图谱表明，根据植物物种形成了不同的群落。最后，在田间条件下，稗草根际的香农-威弗多样性指数和辛普森优势度指数均高于野西瓜苗的根际。这些结果表明，很难将盆栽实验的结果应用于田间条件。这些发现可以用来为未来的研究提供信息，以确定基于盆栽研究的结果，田间场地是否适合植物修复。
