患有急性咽扁桃体炎的成年患者扁桃体培养物中的流感嗜血杆菌和溶血嗜血杆菌。
Haemophilus influenzae and Haemophilus haemolyticus in tonsillar cultures of adults with acute pharyngotonsillitis.
作者信息
Hotomi Muneki, Kono Masamitsu, Togawa Akihisa, Arai Jun, Takei Shin, Ikeda Yorihiko, Ogami Masashi, Murphy Timothy F, Yamanaka Noboru
机构信息
Department of Otolaryngology-Head and Neck Surgery, Wakayama Medical University, 811-1 Kimiidera, Wakayama-shi, Wakayama, 640-8501, Japan.
出版信息
Auris Nasus Larynx. 2010 Oct;37(5):594-600. doi: 10.1016/j.anl.2010.02.005. Epub 2010 Apr 14.
OBJECTIVE
The aim of this study was to evaluate the clinical implication of Haemophilus haemolyticus, one of the closest relative of Haemophilus influenzae, on acute pharyngotonsillitis.
METHODS
We applied polymerase chain reaction (PCR) for 16S ribosomal DNA (rDNA) and IgA protease gene (iga) to distinguish H. haemolyticus and H. influenzae.
RESULTS
Among the 199 Haemophilus spp. isolated from 214 patients with acute pharyngotonsillitis, 52 (24.3%) H. influenzae strains and 23 (10.7%) H. haemolyticus strains were identified by polymerase chain reaction (PCR) for 16S rDNA and IgA protease gene (iga). All H. haemolyticus strains showed hemolysis on horse blood agar and there were no other Haemophilus spp., nonhemolytic H. haemolyticus and H. influenzae variant strains that had absent iga gene. H. hemolyticus showed close genetic relationship with H. influenzae evaluated by pulsed field gel electrophoresis (PFGE). The cases of acute pharyngotonsillitis showing WBC=7000/mm(3) or CRP=8 mg/dl were frequently found among cases with H. influenzae rather than cases with H. haemolyticus.
CONCLUSION
H. haemolyticus is a pharyngeal commensal that is isolated frequently from adults with acute pharyngotonsillitis.
目的
本研究旨在评估溶血嗜血杆菌（流感嗜血杆菌的近亲之一）对急性咽扁桃体炎的临床影响。
方法
我们应用聚合酶链反应（PCR）检测16S核糖体DNA（rDNA）和IgA蛋白酶基因（iga），以区分溶血嗜血杆菌和流感嗜血杆菌。
结果
从214例急性咽扁桃体炎患者中分离出199株嗜血杆菌属菌株，通过对16S rDNA和IgA蛋白酶基因（iga）进行聚合酶链反应（PCR），鉴定出52株（24.3%）流感嗜血杆菌菌株和23株（10.7%）溶血嗜血杆菌菌株。所有溶血嗜血杆菌菌株在马血琼脂上均表现出溶血现象，且不存在其他嗜血杆菌属菌株、非溶血溶血嗜血杆菌和缺失iga基因的流感嗜血杆菌变异菌株。通过脉冲场凝胶电泳（PFGE）评估，溶血嗜血杆菌与流感嗜血杆菌显示出密切的亲缘关系。在流感嗜血杆菌感染的病例中，白细胞计数（WBC）=7000/mm³或C反应蛋白（CRP）=8mg/dl的急性咽扁桃体炎病例比溶血嗜血杆菌感染的病例更为常见。
结论
溶血嗜血杆菌是一种咽部共生菌，常从患有急性咽扁桃体炎的成年人中分离出来。
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