[近红外光谱法测定不同药厂银黄颗粒中黄芩苷的含量]
[Determination content of baicalin in Yinhuang granules from different pharmaceutical factories by near-infrared spectroscopy].
作者信息
Bai Yan, Zhang Wei, Wang Xing
机构信息
Henan University of Traditional Chinese Medicine, Zhengzhou 450008, China.
出版信息
Zhongguo Zhong Yao Za Zhi. 2010 Jan;35(2):166-8. doi: 10.4268/cjcmm20100209.
OBJECTIVE
To determine the content of baicalin in Yinhuang granules fastly from different pharmaceutical factories with near-infrared spectroscopy.
METHOD
Near-infrared diffuse reflectance spectroscopy technology was used to collect NIR spectra of Yinhuang granules. With HPLC anilysis values as reference, the fast determination method of baicalin was established with partial least squares (PLS).
RESULT
The correlation coefficients (R2), the root-mean-square error of cross-validation and the root-mean-square error of calibration (RMSEC) of the calibration model for baicalin were 0.9982, 0.1899 and 0.0514, respectively; the root-mean-square error of prediction (RMSEP) and the average rate of recovery were 0.0802 and 104.27%.
CONCLUSION
The method is accurate, fast and simple, and can be applied for the fast determination of large quantities of numbers of samples.
目的
采用近红外光谱法快速测定不同药厂生产的银黄颗粒中黄芩苷的含量。
方法
运用近红外漫反射光谱技术采集银黄颗粒的近红外光谱。以高效液相色谱分析值为参照，采用偏最小二乘法（PLS）建立黄芩苷的快速测定方法。
结果
黄芩苷校准模型的相关系数（R2）、交叉验证均方根误差和校准均方根误差（RMSEC）分别为0.9982、0.1899和0.0514；预测均方根误差（RMSEP）和平均回收率分别为0.0802和104.27%。
结论
该方法准确、快速、简便，可用于大量样品的快速测定。
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