Kim H S, Chung C H, Kang M S, Ha D B
Department of Molecular Biology, College of Natural Sciences, Seoul National University, Korea.
Biochem Biophys Res Commun. 1991 May 15;176(3):1044-50. doi: 10.1016/0006-291x(91)90388-n.
Okadaic acid was found to block membrane fusion of chick embryonic myoblasts in culture. It also induced morphological change of the cells from bipolar to spherical shape. These effects were dose-dependent, and could be reversed upon removal of the drug from the culture medium. It showed, however, no effect on the induction of muscle specific proteins including tropomyosin and creatine kinase. When okadaic acid was treated to the cell lysates, the phosphorylation state of many proteins significantly increased. These results suggest that the inhibition of myoblast fusion by okadaic acid may be mediated by the increase in the phosphorylation of certain, unknown protein(s) that regulate the fusion process.
冈田酸被发现可阻断培养的鸡胚成肌细胞的膜融合。它还诱导细胞形态从双极状变为球状。这些效应呈剂量依赖性，并且在从培养基中去除药物后可逆转。然而，它对包括原肌球蛋白和肌酸激酶在内的肌肉特异性蛋白的诱导没有影响。当用冈田酸处理细胞裂解物时，许多蛋白质的磷酸化状态显著增加。这些结果表明，冈田酸对成肌细胞融合的抑制可能是由调节融合过程的某些未知蛋白质的磷酸化增加介导的。
