Yokoyama R, Inokuchi T, Takahashi Y, Watanabe I
Department of Anatomy, Kurume University School of Medicine, Japan.
Arch Histol Cytol. 1991 Mar;54(1):59-67. doi: 10.1679/aohc.54.59.
In combination with transmission electron microscopy (TEM), histochemistry for acetylcholinesterase (AChE) and immunohistochemistry for vasoactive intestinal peptide (VIP) and neuropeptide Y (NPY) were carried out on the intraepithelial nerve fibers of the guinea-pig nasal gland. AChE-positive nerve profiles and VIP-immunoreactive nerve profiles were detected in abundance within the epithelium of the glandular acini and within the epithelium of intralobular excretory ducts including the intercalated and striated ducts. Intraepithelial NPY-immunoreactive nerve profiles were also considerably large in number in the nasal gland, but less frequent than the other two types of nerve profiles; furthermore, the NPY-immunoreactive nerve profiles appeared absent within the epithelium of the striated duct. All the intraepithelial nerve varicosities were in close spatial contact with the epithelial cells of the acinus and the duct and also with the myoepithelial cells, which were commonly seen in the acinus and the intercalated duct. Throughout the present study, however, no membranous specializations could be found between the nerve varicosities and the epithelial cells or the myoepithelial cells. The present results suggest an intense and delicate regulation through the collaboration among ACh, VIP and NPY of the secretory activity of the guinea-pig nasal gland, including the emission of acinar secretions into the duct through contraction of the myoepithelium and modification of the secretion contents by the duct epithelium.
结合透射电子显微镜（TEM），对豚鼠鼻腺上皮内神经纤维进行了乙酰胆碱酯酶（AChE）组织化学以及血管活性肠肽（VIP）和神经肽Y（NPY）免疫组织化学研究。在腺泡上皮以及小叶内排泄管（包括闰管和纹状管）上皮内大量检测到AChE阳性神经纤维和VIP免疫反应性神经纤维。鼻腺上皮内NPY免疫反应性神经纤维数量也相当多，但比其他两种神经纤维类型少见；此外，在纹状管上皮内未见NPY免疫反应性神经纤维。所有上皮内神经膨体均与腺泡和导管的上皮细胞以及肌上皮细胞在空间上紧密接触，肌上皮细胞常见于腺泡和闰管。然而，在本研究过程中，未发现神经膨体与上皮细胞或肌上皮细胞之间存在膜特化结构。本研究结果表明，乙酰胆碱、血管活性肠肽和神经肽Y之间相互协作，对豚鼠鼻腺的分泌活动进行了强烈而精细的调节，包括通过肌上皮收缩将腺泡分泌物排入导管以及导管上皮对分泌物成分的修饰。
