Nagarajan S, Tosh C, Murugkar H V, Venkatesh G, Katare M, Jain R, Behera P, Khandia R, Tripathi S, Kulkarni D D, Dubey S C
High Security Animal Disease Laboratory, Indian Veterinary Research Institute, Anand Nagar, Bhopal, Madhya Pradesh, 462 021, India.
Virus Genes. 2010 Aug;41(1):30-6. doi: 10.1007/s11262-010-0477-4. Epub 2010 Apr 16.
In 2008, India experienced widespread outbreaks of H5N1 virus in West Bengal, Tripura, and Assam. The virus was detected in Kamrup district of Assam in November 2008 and subsequently spread to eight more districts. Two Jungle or Large billed crows (Corvus macrohynchos) were found dead in a hospital campus at about 8 km from the foci of initial detection of the virus in the same district. One of the crows was positive for H5N1 avian influenza virus by virus isolation, real time RT-PCR, and RT-PCR tests. Full length sequencing of all the eight segments of the virus was carried out. The phylogenetic analysis indicated that all the eight genes grouped with clade 2.2 viruses and were closely related to the human isolate of Bangladesh and avian isolates from India, Bangladesh, Kuwait, Germany, and Saudi Arabia. The molecular analysis indicated avian receptor (alpha 2,3 sialic acid) specificity, susceptibility to oseltamivir and amantadine group of antivirals and lower pathogenicity to mice.
2008年，印度西孟加拉邦、特里普拉邦和阿萨姆邦爆发了H5N1病毒疫情。2008年11月，在阿萨姆邦的卡姆鲁普区检测到该病毒，随后蔓延至另外八个区。在距同一区病毒最初检测点约8公里处的一家医院校园内发现两只丛林鸦或大嘴乌鸦（大嘴乌鸦）死亡。通过病毒分离、实时逆转录聚合酶链反应（RT-PCR）和RT-PCR检测，其中一只乌鸦的H5N1禽流感病毒呈阳性。对该病毒的所有八个基因片段进行了全长测序。系统发育分析表明，所有八个基因均与2.2分支病毒聚类，并且与来自孟加拉国的人类分离株以及来自印度、孟加拉国、科威特、德国和沙特阿拉伯的禽类分离株密切相关。分子分析表明该病毒具有禽受体（α2,3唾液酸）特异性，对奥司他韦和金刚烷胺类抗病毒药物敏感，对小鼠致病性较低。
