College of Life Sciences, Zhejiang University, Hangzhou 310058, China.
Ecotoxicology. 2010 Aug;19(6):1117-23. doi: 10.1007/s10646-010-0494-z. Epub 2010 Apr 16.
Oxidative stress induced by toxic pollutants is generally responsible for the poor performance of many sequencing batch reactors (SBRs) treating organic wastewater. In this study, the oxidative stress in two SBR systems that dealt with tobacco wastewater was monitored by measuring four popular biomarkers (superoxide dismutase, SOD; catalase, CAT; glutathione, GSH; and malondialdehyde, MDA). In the non-BA (non-bio-augmented) system, more intense oxidative stress was induced by a higher concentration of nicotine in tobacco wastewater, and excessive oxidative stress was induced by 250 mg/l of nicotine at the final stage. However, when a nicotine-degrading bacterial strain Pseudomonas sp. HF-1 was added to the BA (bio-augmented) system, the oxidative stress was significantly reduced compared to the non-BA system (p < 0.01).These results suggested that the oxidative stress was mainly induced by nicotine in the SBR treatment of tobacco wastewater, and that bioaugmentation with strain HF-1 would be a potential strategy to reduce the oxidative stress and thereby improve the performance in SBRs. Additionally, the positive correlation between the nicotine content and CAT, GSH and MDA activity in both systems implied that these parameters can be used as biomarkers for reflecting the performance of SBR treatment of tobacco wastewater, and in monitoring nicotine environmental pollution.
有毒污染物引起的氧化应激通常是许多处理有机废水的序批式间歇反应器（SBR）性能不佳的原因。在这项研究中，通过测量四种流行的生物标志物（超氧化物歧化酶，SOD；过氧化氢酶，CAT；谷胱甘肽，GSH；和丙二醛，MDA）来监测处理烟草废水的两个 SBR 系统中的氧化应激。在非 BA（非生物增强）系统中，烟草废水中较高浓度的尼古丁会引起更强烈的氧化应激，而在最后阶段 250mg/l 的尼古丁会引起过度的氧化应激。然而，当向 BA（生物增强）系统中添加尼古丁降解细菌菌株 Pseudomonas sp. HF-1 时，与非 BA 系统相比，氧化应激明显降低（p < 0.01）。这些结果表明，氧化应激主要是由 SBR 处理烟草废水中的尼古丁引起的，而 HF-1 菌株的生物增强将是降低氧化应激从而提高 SBR 性能的潜在策略。此外，两个系统中尼古丁含量与 CAT、GSH 和 MDA 活性之间的正相关关系表明，这些参数可以用作反映 SBR 处理烟草废水性能的生物标志物，并可用于监测尼古丁的环境污染。
