College of Environmental Science and Engineering, Zhejiang Gongshang University, Hangzhou 310012, China.
Bioresour Technol. 2013 Aug;142:445-53. doi: 10.1016/j.biortech.2013.05.067. Epub 2013 May 24.
Bioaugmentation (BA) using Acinetobacter sp. TW with high nicotine-degrading efficiency was applied in a bioreactor receiving a load of COD (3,200 ± 50 mg/L) and nicotine (1.0 ± 0.1g/L). The results showed that because of the colonization of strain TW, the COD removal was stable at 80-90%, while nicotine removal reached 98% in the BA system. Furthermore, according to PCR-DGGE fingerprinting, compared with the originally activated sludge, more bacteria existed in the BA systems while some bacteria disappeared from the non-BA system. In terms of the quorum sensing, short chain AHLs increased to assist colonization of strain TW, and long chain AHLs were secreted and helped to resist the nicotine toxicity. Compared with the non-BA system, the amounts of ROS, protein carbonyls and 8-OHdG were significant lower in the BA systems, which suggested that strain TW played an important role in eliminating the nicotine toxicity from the bioreactors.
采用具有高效尼古丁降解能力的不动杆菌 TW 进行生物强化（BA），在接受 COD（3200±50mg/L）和尼古丁（1.0±0.1g/L）负荷的生物反应器中应用。结果表明，由于 TW 菌株的定殖，COD 去除率稳定在 80-90%，而 BA 系统中的尼古丁去除率达到 98%。此外，根据 PCR-DGGE 指纹图谱分析，与原始活性污泥相比，BA 系统中存在更多的细菌，而非 BA 系统中有一些细菌消失。在群体感应方面，短链 AHLs 增加以协助 TW 菌株的定殖，而长链 AHLs 被分泌出来帮助抵抗尼古丁毒性。与非 BA 系统相比，BA 系统中的 ROS、蛋白质羰基和 8-OHdG 的含量显著降低，这表明 TW 菌株在消除生物反应器中的尼古丁毒性方面发挥了重要作用。
