Radosevich J A, Siddiqui F S, Rosen S T, Kabat W J
Northwestern University/Veterans Administration, Department of Medicine, Chicago, Illinois 60611.
Br J Cancer Suppl. 1991 Jun;14:86-7.
Adenocarcinomas represent one group of tumours which are seen in the broad spectrum of pulmonary neoplasms. We have produced a monoclonal antibody (44-3A6) which reacts with a 40 kD protein expressed by human primary pulmonary adenocarcinomas and cell lines. Using FACS analysis, this antigen does not appear to be cell cycle specific, and is exposed to the external cell surface. This subcellular localisation has been confirmed using a whole cell, pre-embedding, EM-immunogold labelling technique.
腺癌是在广泛的肺肿瘤中出现的一类肿瘤。我们制备了一种单克隆抗体（44-3A6），它能与人原发性肺腺癌及细胞系表达的一种40 kD蛋白发生反应。通过荧光激活细胞分选术（FACS）分析，这种抗原似乎不是细胞周期特异性的，并且暴露于细胞外表面。使用全细胞、包埋前、电子显微镜免疫金标记技术已证实了这种亚细胞定位。
