[冠心病患者经皮冠状动脉介入治疗前后循环内皮祖细胞的变化]

[Changes of circulating endothelial progenitor cells in patients with coronary heart diseases before and after percutaneous coronary intervention].

作者信息

Luo Ying-Shi, Qian Ju-Ying, Zhang Lei, Ma Jian-Ying, Huang Dong, Fan Bing, Ge Jun-Bo

机构信息

Department of Cardiology, Zhongshan Hospital, Fudan University, Shanghai Institute of Cardiovascular Diseases, Shanghai 200032, China.

出版信息

Zhonghua Xin Xue Guan Bing Za Zhi. 2010 Feb;38(2):126-30.

PMID:20398557

Abstract

OBJECTIVE

To compare circulating endothelial progenitor cells (EPCs) number between normal controls and patients with coronary heart diseases (CHD), and to explore the influence of percutaneous coronary intervention (PCI) on the number of EPCs in patients with CHD.

METHODS

A total of 48 hospitalized patients with CHD were enrolled and divided into three groups, including stable angina pectoris (SAP) group, unstable angina pectoris (UA) group, acute ST-elevation myocardial infarction (STEMI) group. Patients with normal coronary angiography served as controls. The percentage of EPCs in peripheral blood nucleated cells was measured at admission and immediately after and 24 hours after PCI in CHD patients by double-color flow cytometry analysis. EPCs were identified with CD133(+)/VEGFR-2(+).

RESULTS

At admission, the percentage of EPCs in peripheral blood nucleated cells was significantly lower in SAP group (0.043% +/- 0.043%), UA group (0.014% +/- 0.018%) and STEMI group (0.040% +/- 0.036%)than that in the control group (0.111% +/- 0.078%, all P < 0.01). The number of EPCs in UA group was significantly lower than that in the SAP group (P < 0.05). In the UA group, the number of EPCs at 24 hours after PCI (0.054% +/- 0.045%) was significantly higher than before operation (0.014% +/- 0.018%, P < 0.01) and tended to be higher than the value immediately after PCI (0.028% +/- 0.041%, P > 0.05). The before and after PCI EPCs numbers were similar in SAP and STEMI groups (all P > 0.05).

CONCLUSION

The number of peripheral EPCs in patients with CHD is lower than that in normal subjects and negatively related with severity of coronary heart disease. The number of circulating EPCs increased post PCI in patients with unstable angina pectoris.

摘要

目的

比较正常对照组与冠心病（CHD）患者循环内皮祖细胞（EPCs）数量，并探讨经皮冠状动脉介入治疗（PCI）对CHD患者EPCs数量的影响。

方法

共纳入48例住院CHD患者，分为三组，包括稳定型心绞痛（SAP）组、不稳定型心绞痛（UA）组、急性ST段抬高型心肌梗死（STEMI）组。冠状动脉造影正常的患者作为对照组。采用双色流式细胞术分析，于入院时、PCI术后即刻及术后24小时检测CHD患者外周血有核细胞中EPCs的百分比。EPCs通过CD133(+)/VEGFR-2(+)进行鉴定。

结果

入院时，SAP组（0.043%±0.043%）、UA组（0.014%±0.018%）和STEMI组（0.040%±0.036%）外周血有核细胞中EPCs的百分比显著低于对照组（0.111%±0.078%，均P<0.01）。UA组EPCs数量显著低于SAP组（P<0.05）。在UA组，PCI术后24小时EPCs数量（0.054%±0.045%）显著高于术前（0.014%±0.018%，P<0.01），且有高于PCI术后即刻（0.028%±0.041%）的趋势（P>0.05）。SAP组和STEMI组PCI前后EPCs数量相似（均P>0.05）。