Chen Xiao-bin, Pu Xiao-qun, Xie Xiu-mei, Fang Ye-qing, He Jin, Li Xiu-li
Department of Cardiology, Xiangya Hospital, Central South University, Changsha, China.
Zhong Nan Da Xue Xue Bao Yi Xue Ban. 2008 May;33(5):432-7.
To investigate the culture of endothelial progenitor cells (EPCs) from peripheral blood in patients with coronary heart diseases (CHD) before and after percutaneous coronary intervention (PCI), and to observe the cells shape and determine the cell number and proliferation activity.
Ninety-five patients were divided into a CHD group(n=65) and a control group (n=30). The mononuclear cells were isolated from peripheral blood of patients with CHD before, right after and 4 days after PCI by Ficoll-density centrifugation. The isolated cells were cultured in RPMI1640 medium supplemented with VEGF165 and bFGF.EPCs were characterized as adherent cells of double positive for DiL-acLDL uptake and FITC-UEA-I binding by direct fluorescent staining under a fluorescence microscope. The EPCs specific surface mark CD34 and KDR were assessed by fluorescence activated cell sorter analysis. The cell shapes were analysed and the number of colony-forming units(CFU) was counted by phase-contrast microscope.
The number of EPCs reduced in patients with CHD before the PCI, but the cell number was significantly increased in patients with CHD after the PCI, and the number reduced in patients with CHD 4 days after the PCI. How-ever, the number of CFUs did not change in patients before and after the PCI.
PCI can increase endothelial progenitor cells in patients after the PCI; but 4 days after the PCI, this increase will not exist.
研究冠心病（CHD）患者经皮冠状动脉介入治疗（PCI）前后外周血内皮祖细胞（EPCs）的培养情况，观察细胞形态并确定细胞数量及增殖活性。
95例患者分为冠心病组（n = 65）和对照组（n = 30）。通过Ficoll密度梯度离心法从冠心病患者PCI术前、术后即刻及术后4天的外周血中分离单个核细胞。将分离出的细胞培养于添加VEGF165和bFGF的RPMI1640培养基中。通过荧光显微镜下直接荧光染色，将EPCs鉴定为对DiL - acLDL摄取和FITC - UEA - I结合呈双阳性的贴壁细胞。采用荧光激活细胞分选分析评估EPCs特异性表面标志物CD34和KDR。通过相差显微镜分析细胞形态并计数集落形成单位（CFU）数量。
冠心病患者PCI术前EPCs数量减少，但PCI术后患者的细胞数量显著增加，而PCI术后4天患者的细胞数量减少。然而，PCI术前和术后患者的CFU数量没有变化。
PCI术后可增加患者体内的内皮祖细胞数量；但PCI术后4天，这种增加将不复存在。
