Section of Transplantation, Department of Surgery, Transplant Center, University of Chicago, Chicago, Illinois 60637, USA.
ASAIO J. 2010 May-Jun;56(3):241-5. doi: 10.1097/MAT.0b013e3181d7b8e3.
The use of microencapsulation with alginate-poly-l-lysine (PLL) as the encapsulation material has been hampered by overgrowth of collagen around implanted capsules. Studies have shown that poly(ethylene glycol) (PEG) has higher biocompatibility than PLL. In this project, we examined the biocompatibility of PEG in comparison with PLL in the Lewis rat model. Capsules made from either PEG or PLL were implanted into Lewis rats in three anatomical sites: subcutaneous (SC), intramuscular (IM), and intra-epididymis (IE). After 2 or 4 weeks, capsules were retrieved, sectioned, and stained with Sirius Red for analysis of fibrotic overgrowth with ImageJ software. The results were statistically analyzed using either unpaired t test or analysis of variance (ANOVA). PEG demonstrated significantly better biocompatibility in SC, at both 2 and 4 weeks, and IE at 2 weeks (p < 0.0001). No significant differences were found in IM implantation at either time point (p = 0.36) between the two materials. However, there was significantly heavier fibrotic overgrowth around PEG capsules in IE than PLL capsules at 4 weeks (p < 0.01). When compared among the anatomical sites, IM implantation demonstrated significantly less fibrotic overgrowth than other sites for both materials (p < 0.01). In conclusion, PLL and PEG may induce different levels of fibrosis based on anatomical location and duration of implantation.
使用海藻酸钠-聚-l-赖氨酸（PLL）作为包封材料的微胶囊化受到植入胶囊周围胶原过度生长的阻碍。研究表明，聚乙二醇（PEG）比 PLL 具有更高的生物相容性。在这个项目中，我们在 Lewis 大鼠模型中研究了 PEG 与 PLL 的生物相容性。将由 PEG 或 PLL 制成的胶囊植入 Lewis 大鼠的三个解剖部位：皮下（SC）、肌肉内（IM）和附睾内（IE）。2 或 4 周后，取出胶囊，切片，并用 Sirius Red 染色，使用 ImageJ 软件分析纤维化过度生长。使用配对 t 检验或方差分析（ANOVA）对结果进行统计学分析。PEG 在 SC 中的生物相容性在 2 周和 4 周时均明显优于 PLL，在 2 周时的 IE 中也是如此（p < 0.0001）。在 IM 植入的两个时间点（p = 0.36）之间，两种材料之间没有发现显著差异。然而，在 4 周时，IE 中 PEG 胶囊周围的纤维化过度生长明显比 PLL 胶囊更严重（p < 0.01）。当在解剖部位之间进行比较时，对于两种材料，IM 植入显示出明显低于其他部位的纤维化过度生长（p < 0.01）。总之，PLL 和 PEG 可能会根据植入部位和时间的不同，引起不同程度的纤维化。
