LGC Limited, Queens Road, Teddington, Middlesex TW11 0LY, UK.
Analyst. 2010 Jun;135(6):1282-7. doi: 10.1039/c0an00031k. Epub 2010 Apr 17.
A microfluidic-based system was developed for the in situ monitoring of the 7-ethoxyresorufin O-dealkylation (EROD) activity of primary rat hepatocytes by measuring the fluorescent intensity of both cells and their surrounding media. The microfluidic chip was designed to allow the cell suspension and test reagent to be introduced in a layer-by-layer flow format, thereby resulting in a short mixing time by diffusion. A good linear relationship was obtained between the resorufin concentration up to 30 microM and fluorescent intensity over the chip's circular chamber area. The EROD activity was determined with 3-methylcholanthrene (3-MC)-induced hepatocytes. The inhibition effect of alpha-naphthoflavone was also examined on EROD activity resulting in an IC(50) value of 12.98 microM.
建立了一种基于微流控芯片的方法，通过测量细胞及其周围介质的荧光强度，原位监测原代大鼠肝细胞的 7-乙氧基resorufin O-脱烷基化（EROD）活性。该微流控芯片设计成使细胞悬浮液和测试试剂以层层流动的方式引入，从而通过扩散实现短的混合时间。在芯片的圆形腔室内区域，在 30μM 以内的 resorufin 浓度与荧光强度之间得到了良好的线性关系。用 3-甲基胆蒽（3-MC）诱导的肝细胞测定了 EROD 活性。还研究了α-萘黄酮对 EROD 活性的抑制作用，得出 IC（50）值为 12.98μM。
