Phytochemistry and Organic Synthesis Laboratory, University of Bechar, Bechar, Algeria.
Nat Prod Res. 2010 Apr;24(7):669-81. doi: 10.1080/14786410903178376.
Two flavanone glycosides were isolated from the aerial part of Launeae arborescens (Asteraceae), which were identified as hesperidin and naringin. They are the most abundant flavonoids in the edible parts of many species of citrus fruits. In this study, we were interested in the chiral separation and determination of the diastereomerisation barriers of hesperidin and naringin by HPLC methods. The chiral separation HPLC screening of diastereomers of hesperidin and naringin by HPLC methods was accomplished in the normal-phase mode using 11 chiral stationary phases and various n-hexane/alcohol mobile phases. The rate constants and activation energy of diastereomerisation (DeltaG#) of flavanones, naringin and hesperidin were determined, respectively, on Chiralpak IC and Chiralpak IA. The analysis of flavanones isolated in butanol extracts of Launeae arborescens were confirmed by HPLC on Chiralpak IC.
从 Launeae arborescens（菊科）的地上部分分离得到两种黄烷酮糖苷，分别鉴定为橙皮苷和柚皮苷。它们是许多柑橘类水果可食用部分中含量最丰富的类黄酮。在这项研究中，我们对通过 HPLC 方法分离橙皮苷和柚皮苷对映异构体以及确定其非对映异构体的能垒感兴趣。通过 HPLC 方法，在手性固定相上以正相模式，用 11 种手性固定相和不同的正己烷/醇流动相完成了橙皮苷和柚皮苷对映异构体的手性分离 HPLC 筛选。分别在 Chiralpak IC 和 Chiralpak IA 上测定了黄烷酮、柚皮苷和橙皮苷的非对映异构体异构化速率常数（DeltaG#）和活化能。通过 HPLC 在 Chiralpak IC 上对从 Launeae arborescens 的丁醇提取物中分离得到的黄烷酮进行了分析。
