Romar R, De Santis T, Papillier P, Perreau C, Thélie A, Dell'Aquila M E, Mermillod P, Dalbiès-Tran R
Faculty of Veterinary Science, Department of Physiology, University of Murcia, Murcia, Spain.
Reprod Domest Anim. 2011 Feb;46(1):e23-30. doi: 10.1111/j.1439-0531.2010.01617.x.
The primary objective of this study was to compare expression of maternal transcripts in bovine oocyte populations with differential developmental competence: oocytes from prepubertal and pubertal animals; and oocytes from small (3-4 mm) and large (6-10 mm) follicles from pubertal animals. All transcripts were examined in oocytes prior to and after in vitro maturation (IVM). Genes were selected based on their known maternal effect in mouse (ZAR1, STELLA, HSF1, MATER/NLRP5 and its paralogue NLRP9), or their identification as markers of oocyte maturation, either involved in redox metabolism (PRDX1, PRDX2) or meiotic progression (AURKA). Total or polyadenylated forms of the transcripts were followed by reverse transcription coupled to real-time PCR. Six polyadenylated transcripts were found significantly reduced after maturation irrespective of donor age or follicle diameter (p<0.05). Within these six polyadenylated transcripts, ZAR1, NLRP9, HSF1, PRDX1 and PRDX2 were significantly reduced in oocytes from prepubertal animals compared to adult animals (p<0.05). A younger age was also associated with lower abundance (total form) of PRDX2/PRDX1 irrespective of maturation. Total HSF1, PRDX1 and polyadenylated NLRP9 showed a tendency (p values from 0.053 to 0.08) for a higher detection in oocytes from small follicles, thus encouraging further investigation of the follicle diameter model. However, at the present time, follicle size did not significantly affect expression of transcripts examined. In conclusion, this study demonstrates differences in the maternal store of RNA and its regulation during IVM which is dependent on donor age.
青春期前和青春期动物的卵母细胞；以及青春期动物小（3-4毫米）和大（6-10毫米）卵泡中的卵母细胞。在体外成熟（IVM）前后对所有转录本在卵母细胞中进行检测。根据它们在小鼠中已知的母体效应（ZAR1、STELLA、HSF1、MATER/NLRP5及其旁系同源物NLRP9），或者它们被鉴定为卵母细胞成熟的标志物来选择基因，这些标志物要么参与氧化还原代谢（PRDX1、PRDX2），要么参与减数分裂进程（AURKA）。转录本的总形式或聚腺苷酸化形式通过逆转录结合实时PCR进行跟踪。发现六种聚腺苷酸化转录本在成熟后显著减少，与供体年龄或卵泡直径无关（p<0.05）。在这六种聚腺苷酸化转录本中，与成年动物相比，青春期前动物卵母细胞中的ZAR1、NLRP9、HSF1、PRDX1和PRDX2显著减少（p<0.05）。无论是否成熟，较年轻的年龄也与较低丰度（总形式）的PRDX2/PRDX1相关。总HSF1、PRDX1和聚腺苷酸化NLRP9在小卵泡卵母细胞中的检测有升高趋势（p值从0.053到0.08），因此鼓励对卵泡直径模型进行进一步研究。然而，目前卵泡大小并未显著影响所检测转录本的表达。总之，本研究证明了RNA的母体储存及其在IVM期间的调节存在差异，这取决于供体年龄。
