Cell and Tissue Therapy Branch, Center for Biologics, Evaluation and Research, Food and Drug Administration, Bethesda, MD 20892, USA.
Dev Biol. 2010 Jul 1;343(1-2):167-77. doi: 10.1016/j.ydbio.2010.04.003. Epub 2010 Apr 18.
The gene odd paired (opa), a Drosophila homolog of the Zinc finger protein of the cerebellum (Zic) family of mammalian transcription factors, plays roles in embryonic segmentation and development of the adult head. We have determined the preferred DNA binding sequence of Opa by SELEX and shown that it is necessary and sufficient to activate transcription of reporter gene constructs under Opa control in transgenic flies. We have found a related sequence in the enhancer region of an opa-responsive gene, sloppy paired 1. This site also responds to Opa in reporter constructs in vivo. However, nucleotide alterations that abolish the ability of Opa to bind this site in vitro have no effect on the ability of Opa to activate expression from constructs bearing these mutations in vivo. These data suggest that while Opa can function in vivo as a sequence specific transcriptional regulator, it does not require DNA binding for transcriptional activation.
基因 odd 配对（opa）是果蝇小脑锌指蛋白（Zic）家族的哺乳动物转录因子的同源物，在胚胎分割和成虫头部发育中发挥作用。我们通过 SELEX 确定了 Opa 的首选 DNA 结合序列，并表明它足以激活转基因果蝇中受 Opa 控制的报告基因构建体的转录。我们在opa 反应基因 sloppy paired 1 的增强子区域中发现了一个相关序列。该位点在体内报告基因构建体中也对 Opa 有反应。然而，在体外消除 Opa 结合该位点的能力的核苷酸改变对构建体中携带这些突变的 Opa 激活表达的能力没有影响。这些数据表明，虽然 Opa 可以在体内作为序列特异性转录调节剂发挥作用，但它不需要 DNA 结合即可进行转录激活。
